The 2025 edition of the Pharmacopoeia has added a quantitative detection method for β - (1 → 3) - glucan, using high-performance liquid chromatography evaporative light scattering detector (HPLC-ELSD) method (General Rule 0512), calculated as anhydrous glucose, with a content of not less than 50.0%. In the specific operation, a secondary/tertiary amine bonded silica gel chromatography column is required, with acetonitrile water (80:20) as the mobile phase, and glucose is released by acid hydrolysis treatment of the sample. The Ecosil Amino Mix chromatographic column, specifically designed for Poria cocos, can meet the requirements of the 2025 Pharmacopoeia for the determination of Poria cocos content.

According to the latest regulations of Volume 4 of the 2025 edition of the Chinese Pharmacopoeia, for the determination of maltotriose, trehalose, and glucose content, the separation degree between maltotriose, trehalose, and glucose peaks should be ≥ 1.5. The reference solution should be injected three times, and the relative standard deviation (RSD) of the main peak area should not exceed 2.0%. The ECOSIL Sugar Na liquid chromatography column can meet the requirements of pharmacopoeia for the determination of maltotriose, trehalose, and glucose content, and can be used for this project.

According to the fourth part of the 2025 edition of the Chinese Pharmacopoeia, regarding the determination of substances and content related to mannitol, the Ecosil Sugar Ca liquid chromatography column was tested under the chromatographic conditions required by the Pharmacopoeia. In the system suitability solution (1) chromatogram, the separation degree between the mannitol peak and the sorbitol peak was 5.9, which is higher than the standard requirement of 2:0; In the chromatogram of system suitability solution (2), the separation degree of the first chromatographic peak of maltitol and isomaltulose is 2.3, which is higher than the standard requirement of 1.5. The signal-to-noise ratio in system solution (3) is 30, which meets the quantitative limit requirement and can be used for the determination of this project.

The Ecosil Sugar-H liquid chromatography column can meet the requirements of the 2025 edition of the Chinese Pharmacopoeia Part IV for the determination of trehalose content.

GB 5009.253-2016 National Food Safety Standard - Comprehensive Scheme for Determination of Perfluorinated Compounds of Animal Origin. The detection of 17 perfluorinated compounds in samples such as pork, pig liver, eggs, and milk was carried out using a Dema Technology Endeavorsil C18100x2.1mm, 1.8 μ m (Cat. #: 87003) chromatographic column for separation and detection. The recovery rate was between 70-120%, meeting the detection requirements.

According to the national standard GB5009.88-2023, dietary fiber in food was determined using ECOSIL SEC-120, a 7.8 × 300 mm 5 μ m dietary fiber specific chromatographic column. The results showed good peak shape and separation effect.

The 2025 edition of the Chinese Pharmacopoeia categorizes borneol into two types: natural borneol (right-handed borneol) and synthetic borneol. The two have different sources, complex ingredients, and varying pharmacological effects. Natural borneol requires strict separation of enantiomers of levo - and right-handed camptothecin to ensure a purity of ≥ 95% for right-handed camptothecin; To synthesize borneol, it is necessary to control the content of borneol to no less than 55%. The LUBEX Wax gas chromatography column has good reproducibility and excellent separation efficiency for substances such as borneol, which can meet the requirements of pharmacopoeia.

The 2025 edition of the Chinese Pharmacopoeia categorizes borneol into two types: natural borneol (right-handed borneol) and synthetic borneol. The two have different sources, complex ingredients, and varying pharmacological effects. Natural borneol requires strict separation of enantiomers of levo - and right-handed camptothecin to ensure a purity of ≥ 95% for right-handed camptothecin. The LUBEX DEX3 cyclodextrin chiral column adopts a derivatized cyclodextrin chiral stationary phase that matches the requirements of the pharmacopoeia. It has high recognition ability for chiral compounds and achieves baseline separation of levo - and right-handed Longines, which can meet the requirements of the pharmacopoeia.

Compared with the first method of steam distillation, the second method (solid-phase extraction GC-MS/MS method) of GB 5009.26-2023 National Food Safety Standard has the advantages of simple processing flow, high efficiency, and low reagent consumption, and has quickly become a commonly used laboratory testing solution. LUBEX LB-WAX MS domestic gas chromatography column, under this condition, both NDMA and its internal standard NDMA-D6 obtained sharp peak shapes and good separation results, meeting the national standard requirements.