Chromatographic column: Ecosil Amino Mix, 4.6 * 250mm, 5 μ m;

Mobile phase: Acetonitrile water=80-20 (phase composition);

Detector: Evaporative Luminescence Detector (Zhongyi ELSD-8800);

Column temperature: 30 ° C;

Flow rate: 1 mL/min;

Injection volume: 10 μ L.

According to the content determination method of the Chinese Pharmacopoeia, the theoretical plate number of the target peak is greater than 1500, and the separation of impurities around the target substance in the test sample is good. The peak height of the reference substance is 633.492MV, and the noise is below 0.1MV, which meets the detection requirements of this project.