Instrument model: High performance liquid chromatograph

Chromatographic column: ECOSIL SEC-120, 7.8 × 300 mm, 5 μ m, two in series

Mobile phase: primary water

Flow rate: 0.5 ml/min

Column temperature: 80 ℃

Differential detector temperature: 50 ℃

Injection volume: 20 ul

Elution time: 60 minutes

According to the national standard GB5009.88-2023, dietary fiber in food was determined using ECOSIL SEC-120, a 7.8 × 300 mm 5 μ m dietary fiber specific chromatographic column. The results showed good peak shape and separation effect.