Requirements for the determination of trehalose content in the 2025 edition of the Chinese Pharmacopoeia Part IV: According to the chromatographic conditions under the content determination item, take 20 μ l of the reference solution and inject it into the liquid chromatograph. Record the chromatogram, and the signal-to-noise ratio of the peak height of the main component should be greater than 10; Accurately measure 20 μ l of the test solution and 20 μ l of the control solution, inject them into the liquid chromatograph, and record the chromatogram. In the chromatogram of the test solution, except for the solvent peak, the sum of the impurity peak areas before and after the main peak of the test solution shall not exceed 0.5 times (0.5%) of the main peak area of the control solution.

Chromatographic conditions——

Chromatographic column: Ecosil Sugar-H, 7.8 X 300mm, 8 μ um

Mobile phase: water

Detector: Differential Refraction Detector at 35 ° C

Column temperature: 80 ° C

Flow rate: 0.4mI/min

Injection volume: 20 μ L

Using Ecosil SugarH, 7.8X300mm, 8 μ m chromatographic column, under these chromatographic conditions, the results meet the detection requirements.