Chromatography column: Sugar Na, 7.8 * 300mm, 8 μ m

Mobile phase: water

Detection wavelength: Differential Refraction Detector (Waters 2414)

Temperature: 35 ° C

Column temperature: 85 ° C

Flow rate: 0.3mL/min

Injection volume: 20 μ L

Using an Ecosil Sugar Na, 7.8 * 300mm, 8 μ m chromatographic column, and following the standard determination method on page 729 of the Pharmacopoeia, after adjusting the column temperature and flow rate, the separation degree of maltotriose, trehalose, and glucose is greater than 1.5, which can be used for the determination of this project.