Triazophos rapid detection test strip (colloidal gold method)

NegotiableUpdate on 05/06

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Overview

Triazophos Rapid Detection Test Strip (Colloidal Gold Method) $r $n This product uses colloidal gold immunochromatography technology to freeze dry gold labeled antibodies in the detection micropores. Triazophos antigen and sheep anti mouse IgG secondary antibody are separately coated on the detection line (T line) and control line (C line) of nitrocellulose membrane. If the sample contains triazophos, it binds to the colloidal gold labeled triazophos monoclonal antibody during microporous incubation, thereby inhibiting the binding of the gold labeled antibody to the triazophos antigen coated on the nitrocellulose membrane. If the color of the T-line is weaker than that of the C-line or does not show color, that is, the content of triazophos in the sample is equal to or exceeds the detection limit of the product, and vice versa

Product Details

Triazophos rapid detection test strip (colloidal gold method)

Detection Principle
This product uses colloidal gold immunochromatography technology to freeze dry gold labeled antibodies in detection micropores. Triazophos antigen and sheep anti mouse IgG secondary antibody are separately coated on the detection line (T line) and control line (C line) of nitrocellulose membrane. If the sample contains triazophos, it binds to the colloidal gold labeled triazophos monoclonal antibody during microporous incubation, thereby inhibiting the binding of the gold labeled antibody to the triazophos antigen coated on the nitrocellulose membrane. If the color of the T-line is weaker than that of the C-line or does not show color, that is, the content of triazophos in the sample is equal to or exceeds the product detection limit, otherwise it is lower than the product detection limit.

performance metrics
Detected: 0.1mg/kg
Testing time: 9 minutes.

Key sampling varieties
1. GB2763 document contains varieties
Vegetables: cabbage, zucchini.
Fruits: citrus, apple.
2. Other sampled varieties
Vegetables: lettuce, onions, beans, rapeseed, eggplant, potatoes, lettuce, white radish, spinach, winter melon, green pepper, red pepper.
Fruits: grapefruit, grapes, watermelon.

sample pretreatment
1. Take 2 g ± 0.1 g of the crushed sample and add it to a 15 mL or 50 mL centrifuge tube.
2. Measure and add 8 mL of sample universal extraction solution, shake thoroughly and mix well to obtain the test solution

sample testing
1. Mix the liquid in the red micropores and start the reaction at 20-40 ℃ for 3 minutes;
2. Remove the test strip from the test tube and insert it into the red micro hole mentioned above;
3. Start the second step reaction at 20-40 ℃ and time for 6 minutes;
4. Remove the test strip from the micropore, gently scrape off the water absorbing sponge at the bottom of the strip, and interpret the results.

Sample dilution
Remove the test paper tube from the original packaging, open it and take out the required number of red microporous reagents. Dilute different types of samples according to the following methods:

sample variety	GB 2763	dilution method
Vegetables: cabbage, zucchini Fruits: Citrus, Apple	0.1 mg/kg	Add 200 μ L (8-10 drops) of the test solution to the red micropores in sequence, and use a disposable pipette to draw up and down 5-10 times to mix well.
Vegetables: Lettuce, Onion, Beans, Rapeseed, Eggplant, Potato, Oil Wheat Vegetable, White Radish, Spinach, Winter Melon, Green Pepper, Red Pepper Fruits: grapefruit, grapes, watermelon	0.2 mg/kg

result interpretation
（1） Visual inspection
1. Negative (undetected): T-line color ≥ C-line color.
2. Positive (detected): T-line color<C-line color; The T-line does not show color.
3. Invalid: The colors of the C-line and T-line are not visible.