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No. 45-46, Third Street, First Floor, Building B1-B15, C1, D1, E1-E9, Xihuan Road, Panyu District, Guangzhou City
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Guangzhou Yiman Biotechnology Co., Ltd
6-benzyladenine rapid detection test strip
NegotiableUpdate on 05/06
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Overview
6-benzyladenine rapid detection test strip $r $n Scope of application $r $n This product is suitable for on-site or laboratory rapid detection of 6-benzyladenine residues in bean sprouts. $r $n $r $n Detection principle $r $n This product uses colloidal gold immunochromatography technology to freeze dry gold labeled antibodies in the detection micropores. The detection line (T line) and control line (C line) of the nitrocellulose membrane are respectively coated with 6-benzyladenine antigen and sheep anti mouse IgG secondary antibody. If the sample contains 6-benzyladenine, it will bind to the colloidal gold labeled 6-benzyladenine monoclonal antibody during the microporous incubation process
Product Details
6-benzyladenine rapid detection test strip
Scope of application
This product is suitable for on-site or laboratory rapid detection of 6-benzyladenine residues in bean sprouts.
Detection Principle
This product uses colloidal gold immunochromatography technology to freeze dry gold labeled antibodies in the detection micropores. The detection line (T line) and control line (C line) of the nitrocellulose membrane are separately coated with 6-benzyladenine antigen and sheep anti mouse IgG secondary antibody. If the sample contains 6-benzyladenine, it binds to the colloidal gold labeled 6-benzyladenine monoclonal antibody during microporous incubation, thereby inhibiting the binding of the gold labeled antibody to the 6-benzyladenine antigen coated on the nitrocellulose membrane. If the color of the T-line is weaker than that of the C-line or does not show color, that is, the content of 6-benzyladenine in the sample is equal to or exceeds the product detection limit, otherwise it is lower than the product detection limit.
sample pretreatment
1. Take 2 g ± 0.1 g of the crushed sample and add it to a 15 mL or 50 mL centrifuge tube.
2. Measure and add 8 mL of YRSPN sample universal extraction solution, shake thoroughly and mix well to obtain the test solution.
Sample dilution
Remove the test paper tube from the original packaging, open it and take out the required number of red microporous reagents. Dilute different types of samples according to the following methods:
| sample variety | No. 11 of 2015 | dilution method |
| soybean sprouts | Not detected | Add 200 μ L (7-8 drops) of the test solution to the red micropores in sequence, and use a disposable pipette to draw up and down 5-10 times to mix well. |
| mung bean sprouts | Not detected |
sample testing
1. The liquid mixed in the red micropores is reacted at 20-40 ℃ and timed for 3 minutes;
2. Remove the test strip from the test tube and insert it into the red micro hole mentioned above;
3. Start the second step reaction at 20-40 ℃ and time for 6 minutes;
4. Remove the test strip from the micropore, gently scrape off the water absorbing sponge at the bottom of the strip, and interpret the results.
result interpretation
（1） Visual inspection
1. Negative (undetected): T-line color ≥ C-line color.
2. Positive (detected): T-line color<C-line color; The T-line does not show color.
3. Invalid: The colors of the C-line and T-line are not visible.
