Small scale bioreactor

Small scale bioreactor

1Fermentation tank body

1.1Tank structure: Volume ≥2LOff site sterilization, working pressure ≤0.1MpaThe main material is high borosilicate glass and316LStainless steel combination; Equipped with dedicated sampling and discharging valves for fermentation tanks, including air inlet, exhaust outlet, and temperature probe portpHProbe mouthDOStandard interfaces such as probe port, defoaming port, seed transfer port, and feeding port;

1.2Loading coefficient:70%-80%

1.3Air intake filtration: using deep ventilation, maximum ventilation volume:1.5VVMSecondary filtration, accuracy ≤0.2MmSterilization filter, capable of independent sterilization;

1.4Motor: Motor power: ≥0.4KW, Speed:50-1000rpmContinuously stable and adjustable; Control accuracy ±0.5%Maximum speed,resolution1rpm.

1.5Mixing: Top mechanical mixing system, using stainless steel specially heat-treated for mixing shaft, precision machining, with ideal dynamic balance performance, good rigidity, and long-term use without deformation; Adopting adjustable mixing blades, the upper gear is a six blade flat blade and the lower gear is a compression turbine inclined blade. The special design can agitate the material settled at the bottom of the lower head to achieve uniform mixing effect,1Pressure type high-efficiency defoaming paddle, baffle;

2、Small scale bioreactorcontrol system

2.1Temperature control system1set

2.1.1Measurement range:0～150℃；

2.1.2Control range: refrigerant+5℃～±65℃；

2.1.3Control accuracy: ±0.2℃；

2.1.4Resolution: ≤0.1℃；

2.1.5Control content: online temperature value detectionPIDIntelligent control, which can be set according to the temperature required for the fermentation process.

2.1.6Component configuration: temperature sensor, precision heated aluminum plate, and automatic temperature control of external cooling water

2.1.7The temperature band has an alarm function for exceeding the upper and lower limits.

2.1.8Software functions: data curve chart, data report, temperature manual and automatic control modes

2.1.9Temperature program control, at least5Segment program control, temperature value and time can be set arbitrarily.

2.2 PHOnline detection module1set

2.2.1Control mode: fully automatic control,PHValues can be set;

2.2.2Display range:0～14PH；

2.2.3Control range:2～12PH；

2.2.4Resolution: ≤0.01PH；

2.2.5Control accuracy: ±0.02PH；

2.2.6Control content:pHOnline value detectionPIDIntelligent control;

2.2.7Component selection: glass gelpHElectrode detection, capable of high-temperature in-situ sterilization,121℃-30minute And connected with original double shielded signal transmission lines,guaranteepHPrecise control.

2.2.8Execution agency:Microcomputer IntelligencePIDcontrol2The peristaltic pump automatically adds acid or alkali to the flow, makingPHControl the value within the set range,Can be manually or automatically separated(Real time automatic+FTAutomatic mode)Control mode.

2.2.9ThePHEquipped with upper and lower limit over limit alarm function.

2.2.10Software functions: data curve chart, data reportPHManual and automatic control

2.2.11 pH control mode: Three control modes, manual control/real-time automatic control/FT mode automatic control, among which FT control mode is anaerobic fermentation control mode.

2.2.12ThepHProgram control, at least5Segment program control,pHThe value and time can be set arbitrarily.

2.3 DOdetection system1set

2.3.1Detection method: automatic detection;

2.3.2Display range:0-200%；

2.3.3Display accuracy: ±3%；

2.3.4Control content:DOOnline value detection;

2.3.5Component configuration: Dissolved oxygen electrode detection, capable of high-temperature in-situ sterilization,121℃-30minute Using original dual shielded signal transmission lines for connection, ensuringDOAccurate display.

2.3.6System withDOAbnormal alarm function.

2.3.7Can be associated with rotational speed; Automatic addition of oxygen and automatic feeding control of dissolved oxygen have achieved ideal results in controlling dissolved oxygen parameters; Scope:0～100%Control accuracy:±3%Resolution:0.1%；

2.4Pressure and flow control system1set

2.4.1、φ60The surface pressure gauge indicates the pressure in the pipeline, tank, and jacket, and manually controls the tank pressure,range:0-0.25Mpa.

2.4.2The glass rotor flowmeter manually controls the intake flow rate and the ventilation volume can be adjusted.

2.5Feeding system1set

2.5.1Execution mechanism: Constant flow peristaltic pump flow addition.

2.5.2Control mode: automatic flow of materials, detachable/automatic control/Linkage control, automatic mode can provide timed replenishment, quantitative replenishment, segmented program replenishment, at least10Segmental replenishment; Under linkage control, it is possiblepHRelated supplementary materials can be combined withDORelated replenishment can be associated with the rotational speed.

2.6Defoaming system 1set

2.6.1Control mode: defoaming module, fully automatic control;

2.6.2Control accuracy:10-100000Oh?

2.6.3Control content: foam abnormal condition alarm, automatic intermittent processing;

2.6.4Actuator: foam sensor detection, microcomputer automatic control peristaltic pump flow adding defoamer.

2.6.5Control modes: manual and automatic. Can be used as a replenishment pump, at least in replenishment mode10Segment program control, can be combined withDO，pHRelated supplementary materials

2.7 BMP gas production detection function

2.7.1Resolution: ≤6.5ml

2.7.2range0-5000ml/hEspecially in low range, higher accuracy is achieved

2.7.3Measurement error: ±0.01ml

2.7.4Display mode: ≥10Automatic display of inch LCD color touch screen

2.7.5Size: ≤320*280*200mm

2.7.6Detection method: fully automatic digital detection

2.8 BMP instantaneous gas production rate detection function

2.8.1Scope of testing:0～10L/minEspecially in low range, higher accuracy is achieved

2.8.2Resolution: ≤0.01ml/min

2.8.3Detection accuracy: ±0.1%(Full scale)

2.8.4Display mode: ≥10Automatic display of inch LCD color touch screen

2.8.5Size: ≤320*280*200mm

2.8.6Detection method: fully automatic digital detection

2.9Lighting system 1set

2.9.1Adopting a new typeLEDFull white light

2.9.2Light intensity0～100%adjust

2.9.3Standard configuration 16 A fluorescent lamp with adjustable light intensity

2.10Speed control system1Taiwan

Servo motor and driver,speed50-1000rpm±1%Stepless variable frequency speed regulation. Mixing is noiseless, does not generate heat, and does not deform after long-term operation. Fermentation specific sealing ensures no gas leakage during long-term fermentation and guarantees experimental safety.

2.11Lower level fermentation process control system 1set

2.11.1≥10Wide screen color LCD touch screen, programmable controller, one controller can simultaneously detect and control2LThe corresponding parameters of the fermentation tank.

2.11.2Control system functions:

1) It can detect and control the stirring speed, temperature, feeding, defoaming, light intensity, cumulative gas production and instantaneous gas production rate of the fermentation tank, especially for accurate detection of cumulative gas production and instantaneous gas production rate, which can be corrected and controlled in the software engineer interface. Screenshots of software operation screens are required

2) The parameters of each detection and control circuit can be manually set and corrected online on the screen, and the operation has password protection function.

3) The adjustment method is PID, which can meet different control components and control performance, and can achieve manual/automatic disturbance free switching.

4) All kinds of monitoring data can be displayed and recorded in real-time, with display methods including trend lines and charts.

5) The data storage time can be changed arbitrarily, with options such as 1min/5min/10min/30min, etc. A screenshot of the software operation screen is required

2.12 Electrical System and Control Cabinet

2.12.1Using high-quality electrical components, contactors and intermediate relays are selected, and all tank control systems are integrated into the electrical control cabinet.

2.12.2Control cabinet, surface spray coating, anti-static treatment, no interference;

2.12.3Equipment cables (strong and weak), cable trays, cable fixing joints, etc.

2.13 Accessory: Oil free silent air compressor, flow rate ≥40L/minEquipped with pressure reducing valve and oil-water separator

2、 Configuration List:

1.host1set

2.tank body2a

3.Oil free silent air compressor1a