Laboratory microbial fermentation tank

Equipment nameLaboratory microbial fermentation tank(Parameters)

1.working conditions

1.1Laboratory microbial fermentation tankIt can be placed in a regular laboratory or room (tap water is required),220VAC power, drainage system).

2.Technical requirements and configuration

glass fermentation tank

Tank structure:volumecustomizeOff site sterilization, work pressure0.1MpaMain material: high borosilicate glass and316LStainless steel combination; Equipped with dedicated sampling and discharging valves for fermentation tanks, including exhaust ports and temperature probe portspHProbe mouthDOStandard interfaces such as probe port, defoaming port, seed transfer port, and feeding port;

Loading coefficient：70%-80%

Air intake filtrationAdopting deep ventilation with maximum ventilation volume:1.5VVMSecondary filtration, precision0.2MmSartorius sterilization filter, capable of independent sterilization;

electricity Machine:Motor power0.4KW, Turn Speed:50-1000rpmContinuously stable and adjustable; Control accuracy ±0.5%*maximum speed,resolution1rpm；

stir Mixing:Top mechanical mixing system, made of stainless steel specially heat-treated for mixing shaft, precision machined, with ideal dynamic balance performance, good rigidity, and long-term use without deformation; Adopting adjustable mixing blades, the upper gear is a six blade flat blade and the lower gear is a compression turbine inclined blade. The special design can agitate the material settled at the bottom of the lower head to achieve uniform mixing effect,1Pressure type high-efficiency defoaming paddle, baffle;

Vaccination:Adopting a flame inoculation device.

control system

2.1Temperature control system

a、 Measurement range:0～150℃；

bControl range: normal temperature to ±75℃； (Can be fermented at low temperature)

cControl accuracy: ±0.1℃；

dResolution:0.1℃；

eControl content: Temperature value online detection, PID intelligent control, can be set according to the required temperature of the fermentation process.

fComponent selection: temperature sensor, SUS304 stainless steel electrode sheath, bottom heating plate for heating up, solenoid valve for cooling down.

gCharacteristics:

A）PIDThe intelligent control system ensures fast and energy-saving temperature control.

B)JD-WK3Type chassis heating design.

hThe temperature band has an alarm function for exceeding the upper and lower limits.

iSoftware functions: data curve chart, data report, temperature manual and automatic control modes.

2.2 ★PHOnline detection and control system Hamilton/MettlerPHElectrodes and cables)

aControl mode: fully automatic control,PHValues can be set;

bDisplay range:0～14PH；

cControl range:2～12PH；

dResolution:0.01PH；

eTransmitter: JD-BS;

fControl content:pHOnline value detectionPIDIntelligent control;

gComponents:SwitzerlandGlass gel pH electrode detection and cable can be sterilized in place at high temperature, 121 ℃ -30 minutes. Stainless steel electrode sheath, transmitter, and connected with original double shielded signal transmission line to ensure precise pH control.

h、 actuator:Microcomputer IntelligencePIDcontrol2The peristaltic pump automatically adds acid or alkali to the flow, makingPHControl the value within the set range,There are two control modes available: manual and automatic.

iThePHEquipped with upper and lower limit over limit alarm function.

jSoftware functions: data curve chart, data report, PH manual, automatic control.

pHControl mode: Three control modes, manual control/Real time automatic control/FTAutomatic mode control/Among themFTThe control mode is the necessary control mode for fermentation. Provide the software with at least the above functions20 Second operation video

2.3 ★DOonline detection （Hamilton/MettlerDOElectrodes and cables)

aDetection method: automatic detection;

bDisplay range: 0-100% or 0-150%;

c、 Display accuracy: ±0.1%or1%；

d、Transmitter: JD-BS;

eComponent selection:Swiss imported extreme universal dissolved oxygen electrode detection, capable of high-temperature in-situ sterilization, 121 ℃ -30 minutes. The stainless steel electrode sheath and transmitter are connected to the PLC using imported original double shielded signal transmission lines to ensure accurate display of DO.

fThe system has a DO abnormal alarm function.

gThe electrode length is 120mm, the electrode outer diameter is 12mm, and the installation size is 25mm.

2.4Pressure and flow control system 1set

a、 The surface pressure gauge with a diameter of 60 indicates the pressure in the pipeline, tank, and jacket, and manually controls the tank pressure with a range of 0-0.25Mpa.

bThe glass rotor flowmeter manually controls the intake flow rate and the ventilation volume can be adjusted.

2.5supplementMaterial system

aExecution agency:Constant flow peristaltic pump flow addition.

bControl mode: Automatic material feeding, with two control modes: manual and automatic. In automatic control mode, the amount of material fed per unit time can be set.

2.6Defoaming system

aControl mode: defoaming module, fully automatic control;

bControl accuracy:10-100000Oh?

cControl content: foam abnormal condition alarm, automatic intermittent processing;

dActuator: foam sensor detection, microcomputer automatic control peristaltic pump flow adding defoamer.

eControl modes: manual and automatic.

2.7Speed control system1Taiwan

Taiwan, ChinaBC2000DC speed regulator,speed50-1000rpm±1%Stepless variable frequency speed regulation.

2.8.Instantaneous gas production rate detection system

a、Number of channels: 1 channel

b、Detection range: 0-200ml/min, especially with higher accuracy in low range

c、Resolution: 0.01ml/min

d、Detection accuracy: ± 0.1% (full scale)

e、Display mode: LCD color touch screen automatic display

f、Size: 320 × 280 × 200mm

g、Power supply: 220V AC power adapter;

2.9 EFSR gas production rate module0-5000ml/h fully automatic online digital detection

2.10 Lower level fermentation process control system

It can detect and control the stirring speed, temperature, feeding, defoaming, cumulative gas production, and instantaneous gas production rate of the fermentation tank, especially for accurate detection of cumulative gas production and instantaneous gas production rate, which can be corrected and controlled in the software engineer interface. The bidding manufacturer shall provide a copyright registration certificate for the data analysis software to ensure that it is an independent intellectual property protection (non third-party software) of the bidding manufacturer, without any other defects in its rights, and does not infringe any intellectual property or trade secrets of any third party

a、7Wide screen color LCD touch screen, programmable controllerA controller can simultaneously detect and control the corresponding parameters of the fermentation tank;

b、 The biological fermentation process control system consists of an on-site controller, fermentation process control software, sensors, and various executing components.The on-site controller is centered around a programmable controllerIt adopts multi-level expansion and has sufficient system capabilityA/DConversion channelD/AConvert channels and switch inputs and outputs. The on-site controller adopts an industrial grade human-machine interface, and the fermentation system operates normally without an upper computer. This design structure and options better improve the reliability performance of the system.

c、The software control system adopts a fuzzy control structure, which includes a lower level controller and software for on-site signal acquisition and transformation. In terms of control, intelligent PID control is used, with high control accuracy and parameter self-tuning control algorithm.

d、The on-site human-machine operation interface adopts a LCD touch screen, with advanced touch feel and a fully Chinese operation interface. It has functions such as digital setting, quick calibration, data storage, over limit alarm, and password setting.

e、 Controller withUSBSerial port, users can copy the fermentation process parameter values and connect them to a printer on other computers for printing.

f、Functional description of on-site control system:

1）The stirring speed and temperature of the fermentation tank can be adjustedpHValue, detection and control;

2）The parameters of each detection and control circuit can be manually set and corrected online on the screen, and the operation has password protection function.

3）The adjustment method is PID, which can meet different control components and control performance, and can achieve manual/automatic disturbance free switching.

4）All kinds of monitoring data can be displayed and recorded in real-time, with display methods including trend lines and graphs,

5）Each circuit has upper and lower limit and alarm functions.

6）The system has a power-off protection function to protect data during power outages.

7）All operations are carried out on the human-machine interface, implementing visual operations.

8）The system has clock function: fermentation batch date, running time, etc.

9）The alarm can be cut off, which can be short-term or long-term.

10）Display the process of adding acid and alkali; Can achieve curve setting, forpHManual and automatic control of values.

11）Temperature control: It can achieve curve setting and manually and automatically control the temperature value.

12）It has a display of the replenishment process and the total amount of replenishment.

13）It has defoaming function, records high foaming conditions, and displays the defoaming process.

2.11Complete set of electrical system and control cabinet

a、 High quality electrical components (Chint) are used, and contactors and intermediate relays (Schneider) are selected. The control system of all tanks is integrated into the electrical control cabinet.b、 Slope control cabinet, surface spray coating, anti-static treatment, no interference;

cEquipment cables (strong and weak), cable trays, cable fixing joints, etc.

2.12Equipment valvesComplete set of doors, pipes, and pipe fittings

The equipment pipeline system meets the requirements of microbial fermentation, and each pipeline is polished using pollution-free welding. All contact with materials is made of stainless steel pipes with internal and external precision polishing.Accessories include anaerobic/A complete set of aerobic experiments is required.

2.13 auxiliary equipment

Oil free silent air compressor compatible with the equipment