50L fully automatic stainless steel fermentation tank

1. Fermentation tank body part
Tank structure: Capacity 50L, in place one key fully automatic sterilization, design pressure 0.4Mpa, working pressure 0.15Mpa; Main material: 316L stainless steel; The fermentation tank is equipped with observation glasses, lighting fixtures, and standard interfaces such as air inlet, exhaust outlet, jacket upper and lower ports, temperature probe port, pH probe port, DO probe port, defoaming interface, inoculation port, and 2 feeding ports;

Bottom valve: Aseptic diaphragm valve at the bottom of the tank, hygienic, easy to clean, and capable of repeated sterilization, capable of emptying the bacterial liquid inside the tank.

Sampling valve: sterile flange+sanitary grade independent sampling valve, manually opened, constant temperature sterilization, sterile valve group as valve group, there should be no dead corners for cleaning and sterilization

Polishing: Inner polishing accuracy Ra0.4, outer polishing accuracy Ra0.6.

clip Set: Design pressure of 0.3Mpa, material of jacket: 304 stainless steel, used for temperature control and auxiliary sterilization;
Air intake filtration: using deep ventilation, maximum ventilation volume: 2VVM; Secondary filtration, precision 0.01 μ m sterilization filter, capable of independent sterilization;
Motor: Imported AC motor and driver, power 0.55KW, speed: 50-850rpm, continuously adjustable and stable; Control accuracy ± 0.5% * maximum speed, resolution 1rpm;
Mixing: Top mechanical mixing system, double end mechanical seal; Adopting adjustable height mixing blades, single-stage 6-shaped straight blade mixing impeller, single-stage inclined blade mixing impeller, and single-stage serrated defoaming impeller.

Sterilization system: fully automatic one click sterilization, with automatic empty and actual sterilization functions, and supports manual operation function; Sterilization temperature requirement: 121-125 ℃, from the beginning of sterilization to cooling to 30 ℃, air sterilization should not exceed 1 hour, actual sterilization should not exceed 1.5 hours, SIP can be used separately, and the filter can be automatically sterilized separately.
2. Control system
2.1 Temperature control system 1 set
2.1.1. Measurement range: 0-150 ℃;
2.1.2. Control range: refrigerant+5 ℃~65 ℃;
2.1.3 Control accuracy: ± 0.2 ℃;
2.1.4 Resolution: 0.1 ℃;
2.1.5 Control content: Temperature value online detection, PID intelligent control, can be set according to the required temperature of the fermentation process.
2.1.6. Component selection: temperature sensor, SUS316 stainless steel electrode sheath, stainless steel electric heating tube, SUS304 stainless steel constant temperature water tank.
2.1.7. Temperature band over upper and lower limit alarm function.
2.1.8 Temperature segmented control, 5-stage program control. The time interval and temperature control can be set arbitrarily.

2.2 PH online detection and control system 1 set

2.2.1 Control mode: fully automatic control, pH value can be set;

2.2.2. Display range: 0-14PH;

2.2.3. Control range: 2-12PH;

2.2.4 Resolution: 0.01PH;

2.2.5 Control accuracy: ± 0.02PH;

2.2.6 Control content: pH value online detection, PID intelligent control;

2.2.7 Component selection: pH electrode detection of glass gel imported from Switzerland, which can be sterilized in place at high temperature, 121 ℃ -30 minutes. And it is connected with original double shielded signal transmission lines to ensure precise pH control.

2.2.8. Execution mechanism: The microcomputer intelligent PID control system automatically adds acid or alkali to two peristaltic pumps, ensuring that the pH value is controlled within the set range. It can be operated in manual or automatic (real-time automatic+FT mode automatic) control modes.

2.2.9 pH control mode: Three control modes, manual control/real-time automatic control/FT mode automatic control, among which FT control mode is anaerobic fermentation control mode.
2.2.10 pH program control, at least 5 program controls, pH value and time can be set arbitrarily.

2.3 1 set of DO detection system

2.3.1. Detection method: automatic detection;

2.3.2. Display range: 0-200%;

2.3.3. Control accuracy: ± 3%;

2.3.4. Component selection: Swiss imported dissolved oxygen electrode detection, capable of high temperature
Sterilize in place at 121 ℃ for 30 minutes. Using original dual shielded signal transmission lines for connection, ensuring precise display of DO.

2.3.5. The system has a DO abnormal alarm function.

2.3.6. Can be associated with rotational speed; It can be associated with supplementary materials, simultaneously or separately, achieving ideal control of dissolved oxygen parameters; Range: 0~150%, control accuracy: ± 3%; Resolution: 0.1%;

2.4 Ventilation system

2.4.1 Ventilation volume: configured at a ratio of 1:2VVM or higher.

2.4.2. Swiss imported quality flow meters are automatically controlled to adjust the gas flow rate according to process requirements;

2.4.3 The air is filtered into the tank through two stages of pre filter and fine filter, using a sterilization filter with an accuracy of 0.2 μ m.

2.4.4. The air distributor inside the tank evenly distributes gas; The pipeline is equipped with a check valve to prevent material backflow, ensuring safety and greatly extending its service life.

2.5 Tank Pressure System

2.5.1. Swiss imported high-temperature resistant high-precision pressure sensors are used to accurately detect the pressure of the fermentation tank and automatically adjust the tank pressure.

2.5.2. Configure exhaust gas proportional control valve to automatically control tank pressure

2.5.3. Pressure measurement range: 0-0.35Mpa, control accuracy: ± 0.5%.

2.6 One set of feeding system
2.6.1. Execution mechanism: Constant flow peristaltic pump flow addition.
2.6.2 Control mode: Automatic material feeding, with three control modes: manual, automatic, and linkage. In automatic control mode, there are three modes: timed, quantitative, and 10 segment program feeding. In linkage mode, it can be associated with pH and DO

2.7 Bubble suppression system 1 set
2.7.1 Control mode: defoaming module, fully automatic control;
2.7.2. Control accuracy: 10-100000 Ω;
2.7.3 Control content: foam abnormal condition alarm, automatic intermittent processing;
2.7.4 Actuator: foam sensor detection, microcomputer automatic control peristaltic pump flow adding defoamer.
2.7.5 Control mode: manual and automatic control modes. It can be switched to a replenishment pump, with multiple replenishment modes including manual/automatic/sequential control/linkage.

★2.8BMP gas production rate
detection system

2.8.1 Resolution: 6.5ml

2.8.2 Range: 0-5000ml/h, especially with higher accuracy at low ranges

2.8.3 Measurement error: ± 0.01ml

2.8.4 Display mode: 10 inch LCD color touch screen automatic display

2.8.5 Size: 320 * 280 * 200mm

2.8.6 Detection method: Fully automatic digital detection

★ 2.9 BMP instantaneous gas production rate detection system

2.9.1 Detection range: 0~10L/min, especially with higher accuracy in low range

2.9.2 Resolution: 0.01ml/min

2.9.3 Detection accuracy: ± 0.1% (full scale)

2.9.4 Display mode: 10 inch LCD color touch screen automatic display

2.9.5 Size: 320 * 280 * 200mm

2.9.6. Detection method: Fully automatic digital detection

2.10. One set of fermentation process control system for lower computer
2.10.1 10 inch widescreen color LCD touch screen, imported PLC controller, one controller can simultaneously detect and control the corresponding parameters of the fermentation tank;
★ 1) It can detect and control the stirring speed, temperature, pH value, DO value, feeding, defoaming, pressure, lid lifting, gas flow rate, and sterilization of the fermentation tank; Detect BMP gas production rate and BMP instantaneous gas production rate. The BMP background values and coefficients can be modified in the software engineer interface.
2) The parameters of each detection and control circuit can be manually set and corrected online on the screen, and the operation has password protection function.
3) The adjustment method is PID, which can meet different control components and control performance, and can achieve manual/automatic disturbance free switching.
4) All kinds of monitoring data can be displayed and recorded in real-time, with display methods including trend lines and graphs

5) Users can choose the data storage time themselves, which can be 1 minute/5 minutes/10 minutes/30 minutes

3. Supporting equipment
3.1 One steam generator
Electric heating steam generator with automatic water replenishment and shortage protection, pressure switch control, selectable output pressure, power:≥6KW。

3.2 One air compressor

Oil-free and silent≥180L/min,0.7Mpa

3.3 One chiller

1P， Air cooled with automatic water replenishment