Semi automatic/fully automatic medium/high pressure chromatographic separation system

NegotiableUpdate on 01/27

Model

Nature of the Manufacturer: Producers

Product Category

Place of Origin

Online Consult

Overview

The semi-automatic/full-automatic medium pressure/high pressure chromatographic separation system is mainly based on the plant effective ingredients (polysaccharides, saponins, flavonoids, alkaloids, etc.) to be separated, chemical intermediates (amino acids, proteins, nucleic acids, antibiotics, vitamins, etc.), active substances and other substances and the different fillers (macroporous resin, ion exchange resin, gel, silica gel, fiber, etc.) in the chromatographic column:

Product Details

Semi automatic/fully automatic medium/high pressure chromatographic separation system

Column pressure: 3-5 bar Operating temperature: 0 ℃ -60 ℃
Sieve mesh number: 50 mesh -500 mesh, determined according to the thickness of customer filler, for general dextran gel, agarose
Gel, as well as ion exchange resin and macroporous adsorption resin can be blocked.
Column cleaning: Soap water and detergent are suitable cleaning agents for chromatography columns If the chromatography column is contaminated with proteins, it can be cleaned with detergent
Column disinfection: Below 120 ℃, high-pressure disinfection for 20 minutes.
Common materials: glass, polypropylene (PP), stainless steel, rubber lining or PTFE, organic glass: gaskets with silicone, PTFE
Silicone PTFE, rubber sheet
Application areas:
1. Separation of active ingredients from plants and traditional Chinese medicine
2. Refinement of effective ingredients in traditional Chinese medicine
3. Removal of impurities in traditional Chinese medicine
4. Purification of chemical intermediates and synthetic products

The chromatography column is mainly used to separate plant active ingredients (polysaccharides, saponins, flavonoids, alkaloids, etc.)
Intermediate substances (amino acids, proteins, nucleic acids, antibiotics, vitamins, etc.), active substances, and other substances are mixed with the chromatography column
Different fillers (macroporous resin, ion exchange resin, gel, silica gel, fiber and other fillers):
1、 At different affinities,
2、 Under different concentrations of acidic and alkaline solutions,
3、 Under different polar solutions.
4、 Under different pressures and flow rates, different categories or components are hierarchically separated to obtain the effective composition of the plan
The difference between sepure ordinary glass chromatography column and sepureplus medium pressure glass chromatography column is that:
1. The pressure resistance range of the Sepure Plus medium pressure glass chromatography column is 3-6 bar, while the pressure resistance range of the Sepure ordinary glass chromatography column is
1-2bar， The materials and thicknesses of the two are different
2. Sepure Plus medium pressure glass chromatography column with conversion joint (axial expansion function), which can increase pressure and adjust filling inside the column
The height of the material layer, while the ordinary type does not have this function, is the main difference between the two
3. The liquid distribution system of the Sepure Plus medium pressure glass chromatography column is more optimized, complex, and liquid efficient than the conventional type
The distribution effect is more uniform, and the incoming liquid will not impact the packing layer
4. The diameter of sepure ordinary glass chromatography column is as large as 10cm, while the diameter of sepure plus medium pressure glass chromatography column
It can reach 20cm, 25cm, and 30cm, generally used for protein purification, extraction of effective ingredients from natural products, and extraction of nucleic acid plasmids
For experiments involving expensive fillers such as macromolecular separation, it is recommended to use a Sepure Plus medium pressure glass chromatography column
For the convenient installation and ideal separation effect of the Sepureplus medium pressure glass chromatography column, the probability of a successful experiment is much higher
Details
Column structure: glass column, top fasteners, lower fasteners, central layer
Column material: high borosilicate glass (material optional)

SePure plusAll specifications of medium pressure glass chromatography columns are as follows:

produceproductnamecall, name, say, weigh	model	Product Specifications(Inner diameter)mm×lengthcm）
inner diameter12 mm SePure plus medium pressure glass chromatography column (with axial telescopic function) Main technical parameters and column pressure can be increased （3-6bar） (Resistant to organic solvents)	SePure plus1220	12 × 20
	SePure plus1230	12 × 30
	SePure plus1240	12 × 40
	SePure plus1250	12 × 50
	SePure plus1260	12 × 60
	SePure plus1270	12 × 70
	SePure plus1280	12 × 80
	SePure plus12100	12 × 100
	SePure plus12150	12 × 150
inner diameter15 mm	SePure plus1520	15 × 20
	SePure plus1530	15 × 30
	SePure plus1540	15 × 40
	SePure plus1550	15 × 50
	SePure plus1560	15 × 60
	SePure plus1570	15 × 70
	SePure plus1580	15 × 80
	SePure plus15100	15 × 100
	SePure plus15150	15 × 150
inner diameter25 mm	SePure plus2520	25 × 20
	SePure plus2530	25 × 30
	SePure plus2540	25 × 40
	SePure plus2550	25 × 50
	SePure plus2560	25 × 60
	SePure plus2570	25 × 70
	SePure plus2580	25 × 80
	SePure plus25100	25 × 100
	SePure plus25150	25 × 150
inner diameter35 mm	SePure plus3520	35 × 20
	SePure plus3530	35 × 30
	SePure plus3540	35 × 40
	SePure plus3550	35 × 50
	SePure plus3560	35 × 60
	SePure plus3570	35 × 70
	SePure plus3580	35 × 80
	SePure plus35100	35 × 100
	SePure plus35150	35 × 150
inner diameter50 mm	SePure plus5020	50 × 20
	SePure plus5030	50 × 30
	SePure plus5040	50 × 40
	SePure plus5050	50 × 50
	SePure plus5060	50 × 60
	SePure plus5070	50 × 70
	SePure plus5080	50 × 80
	SePure plus50100	50 × 100
	SePure plus50150	50 × 150
inner diameter75 mm	SePure plus7520	75 × 20
	SePure plus7530	75 × 30
	SePure plus7540	75 × 40
	SePure plus7550	75 × 50
	SePure plus7560	75 × 60
	SePure plus7570	75 × 70
	SePure plus7580	75 × 80
	SePure plus75100	75 × 100
	SePure plus75150	75 × 150
inner diameter100 mm	SePure plus10030	100 × 30
	SePure plus10040	100 × 40
	SePure plus10050	100 × 50
	SePure plus10060	100 × 60
	SePure plus10070	100 × 70
	SePure plus10080	100 × 80
	SePure plus100100	100 × 100
	SePure plus100150	100 × 150
inner diameter150 mm	SePure plus15040	150 × 40
	SePure plus15060	150 × 60
	SePure plus150100	150 × 100
inner diameter200 mm	SePure plus20040	200 × 40
	SePure plus20060	200 × 60
	SePure plus200100	200 × 100

Semi automatic/fully automatic medium/high pressure chromatographic separation system