RPMI-1640 sugar free medium (without phenol red)

RPMI-1640 sugar free (phenol red free)

Product Overview

RPMI-1640 was developed by Moore et al. in 1967 at the Roswell Park Memorial Institute (RPMI) in Faroe, New York, USA. RPMI is a type of cell culture medium developed by the institute, and 1640 is the culture medium code. RPMI-1640 is an improved McCoy's 5A medium that uses a bicarbonate buffer system. Unlike most mammalian cell culture media, it has a typical pH 8 formulation. RPMI-1640 medium was originally designed specifically for lymphocyte culture and has now been widely used for the cultivation of various normal and cancer cells, especially suspension cell culture, making it one of the most widely used culture media. RPMI-1640 medium without glucose can adjust the concentration of glucose according to research needs, which is convenient and fast.

Phenol red is used as a pH indicator in the culture medium to continuously monitor the acidity and alkalinity of the culture medium. At low pH values, phenol red makes the culture medium yellow, while at higher pH values, it makes the culture medium purple. At pH values of 7.2-7.4, it is red and suitable for cell culture. However, phenol red also has some drawbacks. Studies have shown that phenol red can mimic the effects of steroid hormones (especially estrogen). Therefore, when using estrogen sensitive cells (such as breast tissue), it is best to use a culture medium that does not contain phenol red. Phenol red can interfere with the detection during flow cytometry analysis. In addition, the presence of phenol red in some serum-free culture medium formulations can interfere with sodium potassium balance.

This product contains various components such as amino acids, vitamins, and inorganic salts required for cell culture, but does not contain proteins, lipids, or any growth factors. Therefore, the product needs to be used with serum or serum-free additives.

Ingredient List

Precautions

1. This product has been filtered and sterilized. When using it, attention should be paid to aseptic operation to avoid contamination;

2. To maintain the excellent performance of this product, do not perform freeze-thaw treatment;

3. This product is only used for scientific research or further research purposes and is not intended for diagnosis and treatment.

Special reminder: All products of our company can only be used for scientific research experiments and are strictly prohibited from being used for clinical medical and other non scientific research purposes! The actual product manual received shall prevail, and the website manual is for reference only.