Large columnar animal DNAOUT

Large columnar animal DNAOUTcharacteristic

This product is used to extract high molecular weight Southern grade genomic DNA from animal cultured cells, animal solid tissues, and anticoagulant blood (fresh or frozen). It has the following characteristics: product and features. This product is an upgraded version of the columnar animal DNAOUT, mainly used for the rapid extraction of genomic DNA from fresh or frozen animal tissues in large quantities. Compared with the columnar animal DNAOUT, it has the following characteristics:

1. The processing capacity is 10-20 times that of column animal DNAOUT.
2. The DNA is pure, and the OD260/280 values of most DNA samples are between 1.8-1.9,No visible RNA contamination, can be directly used for subsequent reactions such as PCR, enzyme digestion, hybridization, etc.
3. The DNA yield is generally between 0.5-2 mg/g (depending on the tissue).
4. Quick, the entire process takes about 20 minutes to operate at room temperature.
5. Safe and non-toxic, this reagent kit is non-toxic to the human body, and has no corrosive or irritating odor.
Usage and Effect
Mix 1-5 g of minced animal tissue with 25 mL of solution A in a self prepared 50 mL plastic centrifuge tube, then incubate at 65 ° C for 5 minutes, centrifuge at room temperature for 5 minutes, transfer the supernatant to a new 50 mL plastic centrifuge tube, extract once with 5 mL (this step can be omitted), transfer to a new 50 mL plastic centrifuge tube, add an equal volume of solution B, mix well, and divide into two columns. After centrifugation, discard the supernatant, wash twice with 25 mL of universal column wash solution, dry shake once, and wash with 1 mL of DNA elution solution to obtain DNA.

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