Nanopore third-generation sequencer PromethION 24

1、 Product Overview

2、 Core Technology

Oxford Nanopore sequencing is based on nanopore single-molecule detection technology. During sequencing, nucleic acid molecules (DNA/RNA) are driven by motor enzymes to pass through the nanopore in a single stranded form, one base at a time. When a base passes through, it changes the current intensity inside the nanopore, and there are differences in the current signals corresponding to different bases (A, T, C, G) and modified bases. The device captures these current changes in real time and uses MinKnow ™ Convert the software into base sequences to achieve direct sequencing of single molecules. This technology does not require PCR amplification, can retain base modification information, and has unlimited read length, enabling real-time data analysis and combining portability and efficiency.

3、 Core performance advantages

Flexible sequencing on demand, no need to wait for sample batches:The sequencing time and content can be independently selected according to the needs, and the flow unit supports independent or collaborative operation, without the need to delay the experiment to gather the sample size; The sequencing capacity of a single flow unit is about 6 times that of MinION flow units, meeting the needs of data of different scales.

High throughput multitasking parallel, adapted to service-oriented laboratories:Supporting multiple DNA or RNA experiments simultaneously, combined with barcode technology, can further increase sample processing capacity, efficiently respond to high-throughput sequencing scenarios, and provide users with professional nanopore sequencing services.

Low deployment threshold, suitable for various laboratories:The equipment occupies a small area and can be installed without the need to renovate existing laboratory facilities; Equipped with comprehensive technical support and simplified deployment process, it can easily adapt to both small research laboratories and large service institutions.

High performance integrated computing, efficient and convenient analysis:Integrating sequencing and analysis functions, equipped with a new generation CPU, 4 NVIDIA A-series GPUs, 60TB SSD storage, and 512GB RAM, with powerful computing power; Pre installed with Ubuntu operating system and MinKnow software, sequencing and real-time analysis can be started immediately upon startup, greatly reducing the result acquisition cycle.

4、 Application Fields

Large scale genome or project sequencingAdapt to complex genome assembly of humans, large animals and plants (such as rice, cattle), or multi-stage coherent large-scale scientific research projects. Its 24 flow units can operate synchronously, with a single flow unitdata outputMax 200Gb， Approximately higher capacity than MinION6 timesIt can quickly cover repetitive sequences and structural variation regions of large genomes, combined with powerful real-time analysis to shorten project cycles.

Group size sequencing researchSuitable for scenarios such as human disease association analysis and population screening in animal and plant breeding. Supporting parallel sequencing of multiple samples, coupled with barcode technology, it can efficiently process hundreds of population samples. 512GB RAM and 4 NVIDIA A-series GPUs ensure fast comparison and mutation detection of large-scale data, providing high-throughput solutions for population genetics research.

High multiplex small genome or targeted region sequencingBatch testing of small genomes such as bacteria and fungi, or analysis of targeted regions such as human cancer driver genes and drug targets. 24 flow units can be split to process different sample groups, with high capacity per unit to meet the deep coverage requirements of targeted areas, without the need for sample batch waiting, suitable for high-frequency small-scale sequencing tasks such as clinical testing and microbial resource library construction.

Whole transcriptome (direct RNA or cDNA) studySupport variable splicing analysis of animal and plant transcriptomes and dynamic monitoring of microbial transcription. Direct RNA sequencing can preserve base modification information, while cDNA sequencing covers low abundance transcripts; The integrated computing capability of the device can analyze full-length transcript sequences in real time, avoiding short read long splicing errors, and assisting in research such as gene expression regulation and disease-related transcript screening.

5、 Equipment specifications

Zebrafish (Beijing) Technology Co., Ltd. provides you with information on instrument parameters, prices, selection, technical principles, etc. For more information, please feel free to contact us.