Nanopore third-generation sequencer PromethION 2 Integrated

1、 Product Overview

Nanopore third-generation sequencer PromethION 2 Integrated(P2i) is a small desktop device designed to run up to two PromethION flow rate units. Each flow unit is independently addressable, meaning that experiments can be conducted simultaneously or run separately. P2i is an independent product that includes enterprise level computing and is responsible for all data collection and base address calling on the device. It also allows users to provide nanopore sequencing services.

Nanopore third-generation sequencer PromethION 2 IntegratedContains two sequencing ports that can be inserted into flow units. This device has the same functionality as the smaller PromethION 2 Solo, but adds * computing capabilities, built-in NVIDIA Ampere GPU, and the ability to call data base calls from two PromethION stream units in real-time. The current chemistry and software support generating up to 290 Gbases * of data from a single PromethION flow unit (* theoretical output Max based on 420 base/second sequencing, lasting for 72 hours).

2、 Core Technology

Oxford Nanopore sequencing is based on nanopore single-molecule detection technology. During sequencing, nucleic acid molecules (DNA/RNA) are driven by motor enzymes to pass through the nanopore in a single stranded form, one base at a time. When a base passes through, it changes the current intensity inside the nanopore, and there are differences in the current signals corresponding to different bases (A, T, C, G) and modified bases. The device captures these current changes in real time and uses MinKnow ™ Convert the software into base sequences to achieve direct sequencing of single molecules. This technology does not require PCR amplification, can retain base modification information, and has unlimited read length, enabling real-time data analysis and combining portability and efficiency.

3、 Core performance advantages

Integrated desktop design with high integration:Adopting a fully self-contained desktop device form, with built-in complete computing modules, there is no need for additional external computers or servers, greatly simplifying the device deployment process, saving laboratory space, and adapting to various scientific research and clinical scenarios.

Powerful computing power+intuitive control, outstanding efficiency:Equipped with high-performance GPU, it provides sufficient computing power support for complex analysis tasks such as real-time base recognition and genome assembly; Equipped with a built-in screen, key information such as sequencing progress and current signals can be directly viewed, making operation and interaction more convenient.

Dual Flow Cell Parallel, Flexible Adaptation:Supports running up to 2 PromethION Flow Cells simultaneously, with the option to choose between single channel independent sequencing or dual channel synchronous startup according to experimental needs. This not only meets the requirements for rapid detection of small batch samples, but also for high-throughput sequencing tasks, making resource utilization more flexible.

Pre configured plug and play, with extremely low threshold:The equipment has completed system pre configuration before leaving the factory, without the need for complex parameter debugging. Simply turn on the power to start the sequencing process, and even non professional technicians can quickly get started, significantly reducing operation and maintenance costs.

High performance storage and computing power support:Equipped with 16TB ultra large capacity SSD, it can locally store massive sequencing data without relying on external storage devices; 64GB high bandwidth RAM ensures smooth data read/write and analysis processes, coupled with NVIDIA A-series GPU, providing powerful computing power for complex real-time base recognition, genome assembly, and mutation analysis, significantly reducing data analysis time.

High definition interaction and flexible adaptation:Equipped with a high-resolution touch screen, it can clearly display key information such as sequencing progress, current signals, and data analysis results, making operation and interaction more intuitive and accurate; Simultaneously supporting integration with existing computer infrastructure, it can be easily operated through local touch screens or integrated into existing laboratory systems, flexibly adapting to different usage habits and workflows.

4、 Application Fields

Whole genome researchSingle channel 8Tb data can achieve deep sequencing of the human whole genome by more than 30 times, long read long assisted T2T (telomere to telomere) complete genome assembly, and analyze complex structural variations;

Deep analysis of metagenomicsHigh yield metagenomic deep sequencing supporting complex environmental samples such as soil and ocean, capable of detecting low abundance (<0.1%) microorganisms and accurately annotating functional genes;

Large scale clinical testingDual channel parallel processing can simultaneously process hundreds of clinical samples (such as virus whole genome sequencing), real-time data analysis facilitates rapid typing and mutation monitoring, and supports public health emergency response;

Research on Animal and Plant BreedingProvide high-throughput long read data for large animal and plant genomes (such as wheat and cattle genomes) to assist in molecular marker development and genetic map construction.

5、 Equipment specifications

*Electronic device function range+5 º C to+40 º C

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