Nanopore third-generation sequencer PromethION 2 Solo

1、 Product Overview

Nanopore third-generation sequencer PromethION 2 SoloAdopting the same technology as MinION and GridION devices. It allows for up to two sequencing experiments to be conducted simultaneously or separately.

Nanopore third-generation sequencer PromethION 2 SoloContains two sequencing ports that can be inserted into PromethION flow units. This device does not have integrated computing capabilities, but can be plugged into GridION Mk1 or a low spec standalone computer. This enables the device to call the data generated by two traffic units in real time. The current chemistry and software support generating up to 290 Gbase * of data from a single PromethION flow unit (* theoretical output Max based on 420 bps sequencing, lasting for 72 hours). The device is equipped with a USB Type-C port for connecting external computing devices (such as GridION devices), so setting up PromethION 2 Solo does not require upgrading facilities.

IICore technology

Oxford Nanopore sequencing is based on nanopore single-molecule detection technology. During sequencing, nucleic acid molecules (DNA/RNA) are driven by motor enzymes to pass through the nanopore in a single stranded form, one base at a time. When a base passes through, it changes the current intensity inside the nanopore, and there are differences in the current signals corresponding to different bases (A, T, C, G) and modified bases. The device captures these current changes in real time and uses MinKnow ™ Convert the software into base sequences to achieve direct sequencing of single molecules. This technology does not require PCR amplification, can retain base modification information, and has unlimited read length, enabling real-time data analysis and combining portability and efficiency.

3、 Core performance advantages

1. FlexibilityThe PromethION 2 Solo, a third-generation nanopore sequencer, can run two independent PromethION Flow Cells simultaneously. It supports single sample dual channel stacking for depth enhancement (such as human whole genome reaching over 100 x) or dual sample parallel sequencing, and is flexibly adapted to high-throughput scenarios with multi sample processing or high depth data requirements, reducing operational complexity.

4、 Application Fields

Whole genome researchSingle channel 8Tb data can achieve deep sequencing of the human whole genome by more than 30 times, long read long assisted T2T (telomere to telomere) complete genome assembly, and analyze complex structural variations;

Deep analysis of metagenomicsHigh yield metagenomic deep sequencing supporting complex environmental samples such as soil and ocean, capable of detecting low abundance (<0.1%) microorganisms and accurately annotating functional genes;

Large scale clinical testingDual channel parallel processing can simultaneously process hundreds of clinical samples (such as virus whole genome sequencing), real-time data analysis facilitates rapid typing and mutation monitoring, and supports public health emergency response;

Research on Animal and Plant BreedingProvide high-throughput long read data for large animal and plant genomes (such as wheat and cattle genomes) to assist in molecular marker development and genetic map construction.

5、 Equipment specifications

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