Nanopore third-generation sequencer MinION

NegotiableUpdate on 05/07

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Overview

MinION, the third-generation nanopore sequencer from Oxford Nanopore Technology (ONT), is a handheld real-time nanopore sequencer that focuses on the portability and real-time analysis capabilities of "sequencing wherever the sample is". It can be used for laboratory testing, field emergency response, and immediate diagnosis and treatment, and is also one of the sequencing equipment that can operate stably in environments such as the International Space Station. It comprehensively optimizes thermal control accuracy, data transmission, and environmental adaptability, providing a cost-effective solution for long read long sequencing.

Product Details

1、 Product Overview

Nanopore third-generation sequencer MinIONMk1DIt is launched by Oxford Nanopore TechnologiesHandheld real-time nanopore sequencerWith the portability and real-time analysis capability of "where the sample is, the sequencing is there", it can be widely used in laboratory routine testing, field emergency sequencing, and instant diagnosis and treatment scenarios. It is currently one of the sequencing equipment that can operate stably in environments such as the International Space Station. This device achieves comprehensive optimization in thermal control accuracy, data transmission, and environmental adaptability, providing a cost-effective solution for long read and long sequencing needs.

2、 Core Technology

Oxford Nanopore sequencing is based on nanopore single-molecule detection technology. During sequencing, nucleic acid molecules (DNA/RNA) are driven by motor enzymes to pass through the nanopore in a single stranded form, one base at a time. When a base passes through, it changes the current intensity inside the nanopore, and there are differences in the current signals corresponding to different bases (A, T, C, G) and modified bases. The device captures these current changes in real time and uses MinKnow ™ Convert the software into base sequences to achieve direct sequencing of single molecules. This technology does not require PCR amplification, can retain base modification information, and has unlimited read length, enabling real-time data analysis and combining portability and efficiency.

纳米孔三代测序仪MinION

3、 Performance advantages

Ultra high data output capabilityA single Flow Cell can achieve powerful data output, with a theoretical maximum data volume of up to 48Gb（Note: This data is based on the ideal conditions of the system running continuously at a rate of 400 bases per second for 72 hours. The actual output may vary due to factors such as library type and operating environment）To meet the needs of large-scale sequencing and provide sufficient data support for research such as whole genome assembly and metagenomic analysis.

Stable active temperature control systemEquipped with efficient active temperature control technology, it can operate stably within a wide temperature range of 10-35 ° C, effectively resisting the interference of temperature fluctuations on the sequencing process. Whether it is a laboratory constant temperature environment or complex scenarios such as the field and space stations, it can ensure the reliability and accuracy of sequencing results.

Humanized appearance designAdopting a brand new architecture design, while optimizing device performance, we focus on improving user experience. The body is equipped with intuitive status LED indicator lights, which do not require complex operations. With just a glance, the sequencing operation progress, equipment working status, and fault prompts can be quickly grasped, simplifying the operation process and reducing the threshold for use.

Portable featuresThe body is compact and lightweight, with an overall size that meets the "palm of your hand" standard, breaking the traditional dependence of sequencing machines on fixed laboratory environments. Whether in routine laboratory operations, field species identification, emergency pathogen detection, or other on-site scenarios, it can be easily carried, achieving flexible applications of "sequencing wherever the sample is".

Real time data flow functionSupport real-time streaming transmission of data, and during the sequencing process, raw data can be synchronously obtained and analyzed without waiting for the complete sequencing process to end, greatly reducing the time for result output and helping to quickly obtain practical analysis conclusions. It is especially suitable for clinical diagnosis, emergency testing and other scenarios with special time requirements.

Unlimited reading length advantageHaving flexible read length adaptation capability, it can sequence nucleic acid molecules of any length from short fragments to ultra long fragments, without being limited by fragment length. This characteristic gives it unique advantages in analyzing complex structural variations in genomes, distinguishing variable splicing subtypes of transcripts, and breaking through the limitations of short read long sequencing technology.

4、 Applicable applications

Nanopore third-generation sequencer MinION Mk1D The output of the flow unit can reach up to 48Gb *, which is very suitable for sequencing small genomes and targeted applications, such as:

Bacterial metagenomicsCovering the diversity of microbial communities, identifying differences in bacterial strains through long-term reading, real-time analysis helps reveal the structure and functional relationship of microbial communities, and reduces experimental costs.
Bacterial isolation sequencingRealize tens of thousands of times coverage of bacterial genomes, long read and long decode complex structures, complete assembly in 1-2 days, and support research on drug resistance genes.
Bacterial whole genome sequencingSupporting high coverage of large bacterial genomes, reducing preference without PCR, portability suitable for field sequencing, and improving experimental timeliness.
Virus amplicon sequencingSupport multi amplicon detection, real-time analysis of virus mutations, results can be obtained within hours, and long reads can reduce the false positive rate of mutations.
Full length 16S rRNA sequencingParallel testing of hundreds of samples, long read and long overlay of full-length 16S rRNA, accurate identification of bacterial strains, and solving the problem of distinguishing closely related species.
Whole exome sequencingSatisfy exon sequencing, long read and long analysis of complex mutations, real-time screening of pathogenic mutations, portable and suitable for remote area samples.
Whole transcriptome (cDNA) sequencingCovering high and low expression genes, obtaining full-length cDNA through long read, splicing free subtyping, and assisting in gene regulation research.
Small transcriptome (direct RNA) sequencingHigh coverage small transcriptome, direct RNA testing warranty decoration, long read long length, portable and suitable for on-site virus detection.

5、 Key hardware parameters and configurations

Dimensions (height x width x depth) (millimeters)	13 x 55 x 125
Weight (g)	130
Rated power Max (W)	7.5
Install port	1 USB-C
Installed software	MinION driver
Sequencing temperature	Sequencing at ambient temperatures ranging from+10 ° C to+35 ° C *

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