Nano particle size and potential analyzer

1、 Product Introduction

NanoCoulter DNano particle size and potential analyzerCapable of multi-dimensional detection of particle size and potential

2、 Technical principle:

There are positive and negative electrodes on both sides of the chip hole in the electrolyte liquid. When a voltage is applied and current passes through the small hole, an "electric induction zone" is generated around the small hole. As each particle passes through the small hole, it will displace an equal volume of conductive liquid, instantly increasing the resistance of the electric induction zone and forming a potential pulse. The instrument obtains characterization data of nanoparticles by accurately measuring and analyzing electrical pulses. The amplitude of the electrical pulse is proportional to the particle size, and the speed at which particles pass through the nanopore (i.e., the width of the resistance pulse signal) is related to the magnitude of the Zeta potential. NanoCoulter can achieve particle characterization technology for simultaneous analysis of single particle size and zeta potential.

3、 Product advantages:

1. No calibration required

Foolish operation, no need for heat engine, no need for calibration. Simply scan the pre made QR code on the detection card to complete all parameter settings.

2. No need to clean

Disposable non-invasive detection card; There is no need to clean the instrument and sample tank before sample testing, and the sample can be directly loaded for testing

3. Intelligent software

Audit tracking function, compliant with 21 CFR part 11; Store complete pulse information for each particle, facilitating multi angle analysis for R&D users

4NanoCoulter DNano particle size and potential analyzerOne detection can simultaneously obtain particle size and potential information

Particle size measurement accuracy comparable to electron microscopy

Single particle Zeta potential detection

4、 Application Cases

extracellular vesicles

Analysis of potential changes after engineering modification of extracellular vesicles

The value of Zeta potential is related to the stability of sample dispersion, and the amount of charge carried by particles directly affects the aggregation state of sample particles. The lower the Zeta potential, the easier it is for particles to aggregate, and the higher the Zeta potential, the more stable the sample tends to be. NanoCoulter detection can accurately detect the particle size distribution and potential changes before and after extracellular vesicle modification.

Virus particles

Adenovirus inter batch differential control

The composition of the culture medium, temperature, pH value, and cell culture method in adenovirus production all affect the efficiency of virus production. NanoCoulterNanoparticle Size AnalyzerReal time monitoring of adenovirus diameter distribution and potential can be performed to quickly evaluate differences between different batches and optimize production processes and parameters.

nanomaterials

The influence of small molecule additives on the zeta of particle suspension systems is significant. When studying the zeta potential of the system, special attention should be paid to the composition of the dispersant environment. With the addition of Tween, Tween molecules adsorb onto the surface of the polymer, shielding the charged groups on the microsphere surface and the surrounding dispersion, resulting in a decrease in the absolute value of the zeta potential.

The most important factor affecting zeta potential is pH. When pH changes, the concentration of protons (H+) in the solution also changes, and the types of ions adsorbed on the particle surface change, leading to changes in particle surface charge and zeta potential.

5、 Technical parameters

1. Particle size

Particle size detection range: 50-2000nm

Particle size measurement accuracy: recovery rate of 100 ± 6%

Particle size measurement accuracy: CV%<3%

2. Potential

Potential measurement range: ± 100mV

4. Sample size: 3-50 μ L (diluted to 200 μ L)

5. Software:

Windows system, operating software in both Chinese and English, providing 3Q certification

Capable of audit trail, compliant with FDA 21CFR Part 11

6. Size: 27 cm x 16.5 cm x 19 cmkg

7. Weight: 8 kg