Nano particle size analyzer (particle size+concentration+potential)

1、 Product Introduction

NanoCoulter G seriesNano particle size analyzer (particle size+concentration+potential)Capable of multi-dimensional detection of particle size, concentration, and potential

2、 Technical principle:

Nano Coulter is a single particle detection method in which each perforated particle produces an instantaneous change in current proportional to the particle volume, with a duration proportional to the particle velocity and thus inversely proportional to the fluid flow velocity. By using a microcurrent detection system to record the electrical pulse signal of each particle, and then calculating it through intelligent analysis software, comprehensive analysis results such as sample particle concentration, particle size, zeta potential, and morphology can be accurately obtained.

3、 Product advantages:

1. No calibration required

Foolish operation, no need for heat engine, no need for calibration. Simply scan the pre made QR code on the detection card to complete all parameter settings.

2. No need to clean

Disposable non-invasive detection card; There is no need to clean the instrument and sample tank before sample testing, and the sample can be directly loaded for testing

3. Intelligent software

Audit tracking function, compliant with 21 CFR part 11; Store complete pulse information for each particle, facilitating multi angle analysis for R&D users

4NanoCoulter G seriesNano particle size analyzer (particle size+concentration+potential)One detection can simultaneously obtain information on particle size, concentration, and potential

Particle size measurement accuracy comparable to electron microscopy

Accurate concentration measurement and precision

Single particle Zeta potential detection

4、 Application Cases

extracellular vesicles

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MISEV2023 evaluates that the RPS measurement results indeed have a very high consistency with TEM data. '

RPS technology, as a "non optical" principle, has become an orthogonal validation method for electron microscopy, flow cytometry, and other techniques.NanoCoulter Nanoparticle AnalyzerProvide precise particle size distribution analysis for EVs research, with a wider particle size LOD (50-800nm) and an extremely wide concentration LOD (5 × 10 ^ 3)⁷-2x10¹¹particles/mL)。

Exploration of methods for isolating extracellular vesicles

The origin of extracellular vesicles is complex and often requires multiple separations to obtain relatively pure extracellular vesicles. Different separation methods have a significant impact on the particle size and concentration of extracellular vesiclesNanoCoulter Nanoparticle AnalyzerCan quickly and accurately determine the advantages and disadvantages of different purification methods.

Study on the purity of extracellular vesicles

Triton X-100 is a surfactant that can cleave the membrane structure of extracellular vesicles. The purity of the sample can be obtained by comparing the changes in particle number of extracellular vesicle samples before and after lysis. The number of particles in the extracellular vesicle sample treated with Triton X-100 significantly decreased, and the purity of the sample was 87.2% (1- after rupture/before rupture) * 100%. This method can quickly achieve quantitative detection of extracellular vesicle purity.

Liposome nanoparticles

Study on the Stability of Liposomes

The stability of different liposome drugs varies greatly, and stability determines the subsequent use of drugsNanoCoulter Nanoparticle AnalyzerThe stability of liposomes can be accurately determined. The following figure shows the concentration changes of two liposome samples after vortex oscillation for different times. It can be seen that sample two has better stability.

LNP particle size composition&potential analysis

Particle size and particle size distribution are important CQA parameters of LNP. The particle size distribution of LNP prepared by different methods varies greatly and is often overlooked in DLS detection.NanoCoulter Nanoparticle AnalyzerIt can truly reflect the particle size distribution of LNP components, and provide the concentration and component ratio within the 'customized' particle size range, as well as the corresponding relationship between the zeta potential and particle size of each nanoparticle.

Virus particles

Adenovirus inter batch differential control

The composition of the culture medium, temperature, pH value, and cell culture method in adenovirus production all affect the efficiency of virus production. NanoCoulterNano particle size analyzerReal time monitoring of adenovirus concentration, particle size distribution, and potential can be performed to quickly evaluate differences between different batches and optimize production processes and parameters.

Aggregation analysis of poxvirus

The storage conditions of viruses have a significant impact on virus aggregation, and the ability to infect decreases correspondingly with more aggregation, NanoCoulterNano particle size analyzerCompared to electron microscopy, the particle size resolution it possesses is an accurate method for obtaining sample aggregation in addition to electron microscopy. As shown in the figure, under two storage conditions, the virus particles in condition 2 were significantly better dispersed.

magnetic nanoparticle

The uniformity of magnetic beads is an important parameter of magnetic beads. Magnetic beads are prone to aggregation, so it is necessary to disperse the particles of magnetic beads through ultrasound. As shown in the figure, three different ultrasound methods are used to disperse nano magnetic beads,NanoCoulter Nanoparticle AnalyzerThe particle size and concentration can be accurately obtained, and the overall dispersibility of ultrasonic method A is better.

latex microspheres

After latex microspheres are coated with antibodies, aggregation often occurs, which affects subsequent experiments and requires ultrasound or other treatments to disperse. useNanoCoulter Nanoparticle AnalyzerBy analyzing the particle size of microspheres before and after coating, the aggregation situation before and after coating can be accurately observed.

5、 Technical parameters

1. Particle size

Particle size detection range: 50-2000nm

Particle size measurement accuracy: recovery rate of 100 ± 6%

Particle size measurement accuracy: CV%<3%

2. Concentration

Concentration measurement range: 1 × 10 ⁶ -1 × 1012 articles/mL

Concentration measurement accuracy: Recovery rate of 100 ± 6%

Accuracy of concentration measurement:CV%<5%

3. Potential

Potential measurement range:±200mV

4. Sample size: 3-50 μ L (diluted to 200 μ L)

5. Software:

Windows system, operating software in both Chinese and English, providing 3Q certification

Capable of audit trail, compliant with FDA 21CFR Part 11

6. Size: 27 cm x 16.5 cm x 19 cmkg

7. Weight: 8 kg