Nano particle size analyzer (particle size+concentration)

1、 Product Introduction

NanoCoulter ENano particle size analyzer (particle size+concentration)Capable of multi-dimensional detection of particle size and concentration

2、 Technical principle:

There are positive and negative electrodes on both sides of the chip hole in the electrolyte liquid. When a voltage is applied and current passes through the small hole, an "electric induction zone" is generated around the small hole. As each particle passes through the small hole, it will displace an equal volume of conductive liquid, instantly increasing the resistance of the electric induction zone and forming a potential pulse. The instrument obtains characterization data of nanoparticles by accurately measuring and analyzing electrical pulses. The amplitude of the electric pulse is proportional to the particle size, and the quantity is proportional to the concentration. Due to the particles passing through the nanopores one by one, true single particle detection has been achieved.

IIIProduct advantages:

1. No calibration required

Foolish operation, no need for heat engine, no need for calibration. Simply scan the pre made QR code on the detection card to complete all parameter settings.

2. No need to clean

Disposable non-invasive detection card; There is no need to clean the instrument and sample tank before sample testing, and the sample can be directly loaded for testing

3. Intelligent software

Audit tracking function, compliant with 21 CFR part 11; Store complete pulse information for each particle, facilitating multi angle analysis for R&D users

4NanoCoulter ENano particle size analyzer (particle size+concentration)One detection can simultaneously obtain information on particle size, concentration, and potential

Particle size measurement accuracy comparable to electron microscopy

Accurate concentration measurement and precision

4、 Application Cases

extracellular vesicles

Recommended Latest Guide to MISEV

MISEV2023 evaluates that the RPS measurement results indeed have a very high consistency with TEM data. '

RPS technology, as a "non optical" principle, has become an orthogonal validation method for electron microscopy, flow cytometry, and other techniques.NanoCoulter Nanoparticle AnalyzerProvide precise particle size distribution analysis for EVs research, with a wider particle size LOD (50-800nm) and an extremely wide concentration LOD (5 × 10 ^ 3)⁷-2x10¹¹particles/mL)。

Study on size distribution and aggregation of extracellular vesicles

The NanoCoulter E series has a large measurement range and can accurately measure the content of large vesicles in extracellular vesicle samples. Paired with chips of different ranges, it can obtain particle concentrations in different particle size ranges. Ultra high sensitivity single particle detection can quickly detect subtle changes before and after sample processing, rapidly advancing the research and development of extracellular vesicles.

Liposome nanoparticles

Study on the Stability of Liposomes

The stability of different liposome drugs varies greatly, and stability determines the subsequent use of drugsNanoCoulter E Nano Particle SizerThe stability of liposomes can be accurately determined. The following figure shows the concentration changes of two liposome samples after vortex oscillation for different times. It can be seen that sample two has better stability.

Analysis of the Proportion of LNP Particle Size Range

Particle size and particle size distribution are important CQA parameters of LNP. The particle size distribution of LNP prepared by different methods varies greatly and is often overlooked in DLS detection.NanoCoulter E Nano Particle SizerIt can truly reflect the particle size distribution of LNP components, and provide the concentration and component ratio within the 'custom' particle size range.

Virus particles

Optimization of adenovirus culture and purification process

The composition of the culture medium, temperature, pH value, and cell culture method in adenovirus production all affect the efficiency of virus production.NanoCoulter E Nano Particle SizerReal time monitoring of adenovirus concentration and particle size distribution can be performed to quickly evaluate differences between different batches and optimize production processes and parameters.

Inter batch difference control of adenovirus

Total particle concentration of adenovirus using different purification methods

Aggregation analysis of poxvirus

The storage conditions of viruses have a significant impact on virus aggregation, and the ability to infect decreases correspondingly with more aggregation, NanoCoulterNano particle size analyzerCompared to electron microscopy, the particle size resolution it possesses is an accurate method for obtaining sample aggregation in addition to electron microscopy. As shown in the figure, under two storage conditions, the virus particles in condition 2 were significantly better dispersed.

magnetic nanoparticle

The uniformity of magnetic beads is an important parameter of magnetic beads. Magnetic beads are prone to aggregation, so it is necessary to disperse the particles of magnetic beads through ultrasound. As shown in the figure, three different ultrasound methods are used to disperse nano magnetic beads,NanoCoulter Nanoparticle AnalyzerThe particle size and concentration can be accurately obtained, and the overall dispersibility of ultrasonic method A is better.

latex microspheres

Latex microspheres are coated with antibodies and specifically bind to antigens in the sample, causing changes in the turbidity of the solution. The particle size of the microspheres directly affects the sensitivity of the immunoturbidimetric reagent. NanoCoulter accurately distinguishes the particle size and concentration changes of microspheres before and after the process. Uniformly dispersed and stable bare microspheres are prone to aggregation during modification and coating processes due to changes in surface properties. Usually, latex microspheres need to be dispersed by ultrasound, and the particle size distribution is checked by NanoCoulter to screen for suitable dispersion conditions.

5、 Technical parameters

1. Particle size

Particle size detection range: 50-2000nm

Particle size measurement accuracy: recovery rate of 100 ± 6%

Particle size measurement accuracy: CV%<3%

2. Concentration

Concentration measurement range: 1 × 10 ⁶ -1 × 1012 articles/mL

Concentration measurement accuracy: Recovery rate of 100 ± 6%

Concentration measurement accuracy: CV%<5%

3. Sample size: 3-50 μ L (diluted to 200 μ L)

4. Software:

Windows system, operating software in both Chinese and English, providing 3Q certification

Capable of audit trail, compliant with FDA 21CFR Part 11

5. Size: 27 cm x 16.5 cm x 19 cmkg

6. Weight: 8 kg