Corticosteroid induced osteoporosis animal model

Corticosteroid induced osteoporosis animal model

Corticosteroid induced osteoporosis animal model

1、 Model mechanism‌

Corticosteroids inhibit osteoblast function, reduce collagen synthesis, lead to reduced bone matrix formation, and cause calcium and phosphorus metabolism disorders, thereby inducing osteoporosis
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‌2、 Common animals and strains‌

1. ‌rat‌

• ‌strainSD (Sprague Dawley), Wistar rats
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• ‌age3-12 months old (avoid being over 24 months old to eliminate age interference)
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• ‌characteristicLow cost, good repeatability, but lacking the Harvard system
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2. ‌mouse‌

• ‌strainC57BL/6, etc
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• ‌advantageGene modification is convenient and suitable for mechanism research
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3. ‌Rabbit/Sheep‌

• ‌rabbitSuitable for orthopedic implant research, but with limited availability of cancellous bone
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• ‌sheepCortical bone is close to humans, but it has high cost and long cycle
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‌3、 Modeling method‌

‌1. Drug selection and dosage‌

‌2. Administration method‌

• ‌Injection method(Commonly used): Intramuscular or subcutaneous injection
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• ‌Oral administration/sustained-release implantation: Suitable for specific research needs
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‌3. Auxiliary acceleration methods‌

• ‌Low calcium dietCan accelerate bone loss
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• lackEnhance model performance
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‌4、 Characteristics and limitations of the model‌

1. ‌advantage‌

• High success rate and easy operation
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• Simulated clinical secondary osteoporosis caused by glucocorticoids
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2. ‌shortcoming‌

• May cause bone necrosis, immune suppression, and even death
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• Reversible bone loss effect after discontinuation of medication, not suitable for bone resorption inhibition research
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‌5、 Model evaluation indicators‌

1. ‌Bone mineral density (BMD): Detection through DXA or micro CT
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2. ‌Organizational MorphologySparse and disordered arrangement of bone trabeculae (HE staining)
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3. ‌serum marker‌：

• Bone formation: ALP, osteocalcin ↓.

• Bone resorption: TRAP, CTX ↑
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‌6、 Precautions‌

• ‌Animal ageChoose the stable bone mass period (3-12 months old)
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• ‌Dose controlTo avoid serious side effects caused by excessive dosage
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• ‌Comparison settingsSimultaneous injection of physiological saline control group is required

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