Animal model of injecting autologous whole blood into the right basal ganglia of rats

Animal model of injecting autologous whole blood into the right basal ganglia of rats

Animal model of injecting autologous whole blood into the right basal ganglia of rats：

1、 Experimental animal selection

• ‌Commonly used animalsMale SD or Wistar rats (weight 250-350g),

• ‌requirementNo coagulation dysfunction or infection, SPF grade feeding environment.

2、 Preoperative preparation

1. ‌anesthesia‌

• Gas anesthesia
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• After anesthesia, fix the prone position on the stereotaxic device and adjust the level of the anterior fontanelle and skull plane.

2. ‌blood collection‌

• ‌部位Tail vein, femoral artery or carotid artery
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• ‌blood collection volume50-100 μ L for rats (20-50 μ L for mice), requiring heparinization for anticoagulation treatment
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3、 Surgical operation

1. ‌Positioning and Drilling‌

• ‌coordinate0.2-1.0mm behind the anterior fontanelle, 3.0mm beside the sagittal suture, 5.5-6.0mm below the skull surface (pre experimental calibration required)
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• ‌drillingDental drill hole (with a diameter of about 1mm) to avoid damaging the dura mater
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2. ‌Blood injection‌

• ‌Injection speed≤ 2 μ L/min, divided injection (pause for 10 minutes each time to prevent reflux)
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• ‌Injection volumeRats typically consume 50-100 μ L (mice consume 20-50 μ L)
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• ‌Post injection treatmentLeave the needle in place for 2-15 minutes to ensure blood diffusion, then slowly withdraw the needle
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4、 Postoperative treatment

• Wax sealing of bone holes, suturing of scalp, single cage feeding and insulation
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• Monitor vital signs, behavioral changes, and neurological deficits (such as Longa score, foot error test)
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5、 Model features

• ‌pathological changesHematoma compression causes cerebral edema (peak within 24 hours), inflammatory response, and gliosis
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• ‌applicationStudy on the mechanism of edema and hematoma absorption after cerebral hemorrhage and evaluation of drug efficacy
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6、 Precautions

• ‌Model consistencyStrict control of injection speed, anticoagulation treatment, and positioning accuracy is required
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• ‌Code of EthicsFollowing the "3R principle", reducing animal suffering and optimizing experimental design

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