Collagen+adrenaline induced pulmonary embolism animal model

Collagen+adrenaline induced pulmonary embolism animal model

Collagen+adrenaline induced pulmonary embolism animal model

1. ‌Experimental animal selection‌

• ‌mouse: Commonly used C57BL/6 strain, 8-12 weeks old, weight 20-25g
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• ‌ratLike Sprague Dawley or Wistar strains, weighing 200-250g
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• ‌rabbitFor special experimental needs, weighing 2.5-3.0kg.

2. ‌Drug preparation and dosage‌

• ‌collagenThe usual concentration is 2-4mg/ml, and the dose is 430 μ g/kg (for mice) or 5-10ml/kg (for rats)
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• ‌adrenaline: Concentration of 0.1-0.2mg/ml, dosage of 20 μ g/kg (mice) or 0.1-0.2ml/kg (rats)
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• ‌solventDissolve in PBS buffer, ready to use
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3. ‌modeling method‌

• ‌Injection method‌：

• Mouse: Tail vein injection
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• Rats: injection via tail vein or internal jugular vein
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• Rabbit: jugular vein injection
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• ‌operating steps‌：

1. Inject collagen solution first and observe for 5-10 minutes.

2. Inject adrenaline solution again and slowly push it in
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4. ‌Model evaluation indicators‌

• ‌clinical manifestationsShortness of breath, flapping of nasal wings, cyanosis, reduced activity, etc
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• ‌Survival rate and timeRecord the time of spontaneous death, and determine death if breathing stops for 1 minute
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• ‌Pathological examinationHE staining shows pulmonary thrombosis and alveolar wall congestion and edema
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• ‌HemodynamicsMonitor blood pressure, heart rate, pulmonary artery pressure, etc. (optional)

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