Animal model of renal failure in rats

Animal model of renal failure in rats

Animal model of renal failure in rats

1Acute renal failure (ARF) model‌

1. ‌Ischemia-reperfusion model‌

• ‌methodAfter clamping the bilateral renal arteries for 45-60 minutes (or unilateral renal artery+contralateral nephrectomy), restore blood flow.

• ‌characteristicSimulated ischemic necrosis of renal tubular epithelial cells, characterized by increased levels of serum creatinine (Scr) and blood urea nitrogen (BUN), as well as increased MDA content in renal tissue
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• ‌applicationResearch on Oxygen Free Radical Damage and Drug Protection Mechanisms
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2. ‌Drug induced model‌

• ‌Glycerol modelIntramuscular injection of 50% glycerol (10 mL/kg) induces rhabdomyolysis and renal tubular obstruction
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3. ‌Lipopolysaccharide (LPS) model‌

• ‌methodIntravenous injection of LPS (100 mg/kg) leads to systemic inflammatory response and reduced renal blood flow

IIIModel selection suggestions‌

• ‌Acute studyIschemia reperfusion (clear mechanism)
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• ‌Chronic research5/6 nephrectomy (high stability) or adenine (pathology similar to human gouty kidney)
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• ‌Ethical considerationsPostoperative analgesia is required, and the dosage of anesthesia should be controlled
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4evaluation metric‌

• ‌Biochemical testing‌： Scr ≥ 2 mg/dL、BUN ≥ 50 mg/dL
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• ‌Pathological examinationHE staining to observe renal tubular necrosis, Masson staining to evaluate fibrosis
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• ‌Functionalism24-hour urinary protein and glomerular filtration rate (GFR)