Column shaped mollusk DNAOUT

Column shaped mollusk DNAOUTcharacteristic
This product is a reagent kit specifically designed for extracting genomic DNA from various mollusk tissues. The principle is based on the cationic descaling agent CTAB, which specifically binds to genomic DNA under appropriate ionic strength conditions to form a precipitate, and then further purified using silica gel membrane technology. This product has the following characteristics:
1. Suitable for various mollusks rich in polysaccharides that are difficult to extract using conventional methods, including snails, shellfish, squid, octopuses, and snails.
2. The integrity of the extracted genomic DNA,The longest one can reach a length of generally 20-50kb.
3. The DNA is pure, with an OD260/280 of around 1.9 and a DNA segment size of around 30-50 Kb, which can be directly used for PCR, enzyme digestion, hybridization, etc.
4. Easy to operate, the entire process takes about 30 minutes.

5. Safe and non-toxic, this reagent kit is non-toxic to the human body, and has no corrosive or irritating odor.
6. Affordable and high-quality, comparable in quality to similar foreign products, but at a cheaper price.
Usage and Effect
Mix 0.1-0.3g of soft animal tissue small pieces with 1 mL of solution A preheated at 65 ℃ in a 15mL plastic tube using a Polytron homogenizer for 1 minute, keep at 65 ℃ for 5 minutes, centrifuge for 3 minutes, transfer the supernatant to a new centrifuge tube, add an equal volume of solution B, mix well, ice bath for 5 minutes, centrifuge for 3 minutes, transfer the supernatant to a new centrifuge tube, add 0.2 mL (self prepared), mix well and centrifuge for 3 minutes, transfer the supernatant to a new centrifuge tube, add 1.5 times the volume of solution C, mix well, and transfer the mixture to a centrifugal adsorption column,Leave at room temperature for 5 minutes, centrifuge for 1 minute, discard the penetration solution, wash twice with 10 mL of universal column wash solution, dry shake and wash with 100 uL of DNA eluent 2.0 to obtain DNA.

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