Animal model of skin necrosis

1、 Model building method

1. ‌Mechanical damage method‌

• ‌Complete skin excisionUsing a punch to create a circular full-thickness skin defect with a diameter of 1.5-4cm on the back of an animal, reaching a depth of the subcutaneous tissue, suitable for studying the secondary healing process
  

• ‌Tangent cuttingCut the skin with a sharp blade to form a linear incision (length 2-4cm), which can be controlled for primary or secondary healing by suturing or not
  

2. ‌Low temperature frostbite method‌

• Liquid nitrogen pre cooled metal probe (-196 ℃) in contact with the skin surface for 90 seconds induces local ischemic necrosis and is suitable for studying tissue repair after frostbite
  

3. ‌Chemical damage method‌

• Using acidic/alkaline substances or proteolytic enzymes to disrupt skin structure and simulate necrosis caused by chemical burns
  

2、 Key points of model selection

• ‌animal speciesRats (200-250g) or miniature pigs are more commonly used, with the latter having skin structures closer to humans
  

• ‌Wound localizationIt is recommended to choose both sides of the back and spine to avoid interference from animal licking
  

• ‌Comparison settingsA sham surgery group (only incision without damage) needs to be established to eliminate the influence of the operation itself5
  

3、 Evaluation indicators

1. ‌Macro Perspective‌

• Record wound healing rate, changes in necrotic area, and healing time4
  

2. ‌histological analysis‌

• HE staining observation of inflammatory infiltration, granulation tissue formation, and re epithelialization degree in necrotic areas7
  

3. ‌Molecular testing‌

• Analyze the expression of inflammatory factors (such as TNF - α, IL-6) or repair related proteins in necrotic surrounding tissues

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