Animal model of silicosis in mice

Animal model of silicosis in mice

Animal model of silicosis in mice

Common mouse strains and basic parameters

C57BL/6 strain mice commonly used in silicosis researchThe specific parameters include:

• ‌age10-12 weeks old

• ‌weight‌： 25-30g

• ‌gradeSPF level

• ‌genderUsually male is selected

Main molding methods

Nasal instillation method of silica suspension

1. ‌solution preparation‌：

• Low dose group: 10 mg/mL SiO ₂ physiological saline suspension
  

• High dose group: 20 mg/mL SiO ₂ physiological saline suspension
  

2. ‌operating procedure‌：

• Starting the experiment after 7 days of adaptive feeding
  

• Drip 50 μ L of corresponding solution through the nose once a day
  

• Continuous administration for 28 days
  

3. ‌Model characteristics‌：

• Obvious silicon nodules formed around the bronchi
  

• Dose-dependent alveolar septal thickening
  

• Structural disorder of lung tissue with inflammatory cell infiltration
  

Tracheal instillation method

• Post anesthesia positioning of trachea
  

• Use an indwelling needle for airway instillation
  

• Postoperative nutritional supplementation (if frozen)
  

Model evaluation system

Pathological examination

1. ‌H&E staining‌：

• Display the degree of inflammatory cell infiltration
  

• Assess the damage to alveolar structure
  

2. ‌Masson staining‌：

• Detecting the distribution of fibrotic lesions
  

• Quantitative collagen deposition degree
  

Imaging Evaluation

• High resolution CT can clearly display lesions
  

• Dynamic observation of fibrosis progression
  

Molecular biology indicators

• Changes in microRNA expression such as miR-23a-3p
  

• Levels of inflammatory factors (TNF - α, IL-6, etc.)
  

Model application field

Research on the pathogenesis

• Mechanism of silica induced fibrosis
  

• The relationship between cell apoptosis and lung injury
  

Drug evaluation

1. ‌traditional medicine‌：

2. (signed)Verification of therapeutic effect

3. Dosage regimen: 100-200mg/kg gavage for 28 days
   

4. ‌natural products‌：

• Screening of anti fibrotic components such as Brucea Javanese Bitter Alcohol

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