Animal model of brain injury

NegotiableUpdate on 05/06

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Overview

Animal models of brain injury are important tools for studying the pathological mechanisms and treatment strategies of traumatic brain injury (TBI). The following are common types of models and construction methods:

Product Details

　　Animal model of brain injury

　　1、 Main Model Types

1. Mechanical impact model

-Free fall strike method: Use a 40g metal rod to strike the rat's parietal bone window from a height of 20cm, causing local contusion and laceration

-Controllable Cortical Impact (CCI): Set impact parameters through precision equipment (such as velocity 5m/s, depth 3.5mm, duration 500ms)

-Fluid impact model (FPI): Simulating non penetrating concussion through liquid pulses, suitable for diffuse injury research

2. Young animal model

-For the study of pediatric brain injury, it is necessary to consider the differences in metabolism and pharmacodynamics between juveniles and adults

　　2、 Experimental procedure (taking SD rats as an example)

1. Preoperative preparation

-Select 8-week-old male SD rats (250-300g)

-After anesthesia, shave the hair on the top of the head, disinfect and craniotomy (5mm diameter bone window)

2. Injury induction

-Falling method: locate the impact point 1.5mm behind Bregma and 2.5mm beside the sagittal suture

-CCI method: Typical parameters are impact depth of 1.5mm and velocity of 4.5m/s

3. Postoperative management

-Monitor the awakening status after suturing the incision

-Use heating pads for insulation and administer antibiotics preventively

　　3、 Evaluation indicators

1. Neurological Function Score (mNSS)

-0 points normal, 1-6 points mild injury, 13-18 points severe injury

-Includes tests for movement, sensation, and reflexes (such as assessment of limb coordination)

2. Organizational analysis

-Compare the extent of brain tissue damage between the model group and the control group

-Detecting the association between inflammatory response and neurodegenerative diseases

　　4、 Application direction

-Study on the pathological association between TBI and Alzheimer's disease

-Evaluate neuroprotective drugs or AI assisted treatment options
　