TUNEL Cell Apoptosis Detection Kit (Green Fluorescence)

NegotiableUpdate on 05/06

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Overview

The KTA2010-TUNEL apoptosis detection kit (green fluorescence) 1516/20T $r $n developed TdT enzyme and convenient experimental protocol, suitable for fluorescence enzyme-linked immunosorbent assay, fluorescence microscopy, and flow cytometry, respectively. $r $nTUNEL Cell Apoptosis Detection Kit (Green Fluorescence)

Product Details

TUNEL Cell Apoptosis Detection Kit (Green Fluorescence)

TUNEL Cell Apoptosis Detection Kit (Green Fluorescence)

Product Information

Product English Name	TUNEL Apoptosis Detection Kit (Green Fluorescence)
Experimental suggestions	AbbeyTUNEL Cell Apoptosis Detection Kit (Green Fluorescence)Labeling deoxyribonucleotide fluorescein conjugates onto the 3 '- end of DNA gaps using deoxyribonucleotide end transferase (TdT)

Product attributes

Kit components	TdT enzyme • Balanced buffer solution (5X) • Nucleotide labeling mixture
Features&Advantages	The optimized experimental plan is applicable to fluorescence enzyme-linked immunosorbent assay (ELISA), fluorescence microscopy, and flow cytometry. Based on the widely used FITC channel (Ex/Im=490nm/520 nm), detection is convenient.
Precautions during use	The reaction buffer contains dimethylarsinate and cobalt chloride, which are highly toxic chemicals. Immediately rinse with plenty of water after skin contact. If there is an accident or discomfort, please seek medical attention immediately. Please do not eat or smoke while using.
Save Suggestions	It is recommended to store the goods at -20 ° C after receiving them, with a minimum shelf life of 6 months. Once opened, please refer to the best storage conditions for each component in the instruction manual for storage.
transport conditions	Blue Ice Transport
Warning	The products listed in this article are for research purposes only and are not suitable for human or clinical diagnosis. The applications recommended by our product are not intended to violate any license by using our product. We do not assume any responsibility for any infringement or other violations that may occur when using this product.

Additional information

background

In cell apoptosis, chromosome DNA double or single strand breaks produce a large amount of sticky 3 '- OH ends. Under the action of deoxyribonucleotide end transferase (TdT), derivatives formed by deoxyribonucleotide and fluorescein, peroxidase, alkaline phosphatase or biotin can be labeled onto the 3' - end of DNA to detect apoptotic cells. This method is called terminal deoxyribonucleotide transferase mediated nick end labeling (TUNEL). Due to the fact that normal or proliferating cells have almost no DNA breaks, there is no formation of 3 '- OH and they are rarely stained. As a result, TUNEL has become a commonly used method for detecting DNA fragmentation (apoptosis).

Images and explanations

Fig. Fluorescence images using Abbkine TUNEL Apoptosis Detection Kit (Green Fluorescence) in HeLa cells with the treatment of 10 U/mL Dnase I for 10min.