Annexin V-AbFluor488 Cell Apoptosis Detection Kit

NegotiableUpdate on 05/06

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Nature of the Manufacturer: Producers

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Overview

KTA0002 AnnexinV AbFluor488/PI Double Stained Cell Apoptosis Detection Kit 639/2T $r $nAbFluor488 is a substitute for FITC, which not only has better fluorescence intensity and photostability, but also is not affected by pH $r $nAnnexin V-AbFluor488 Cell Apoptosis Detection Kit

Product Details

Annexin V-AbFluor488 Cell Apoptosis Detection Kit

Product Information

Product English Name	Annexin V-AbFluor™ 488 Apoptosis Detection kit
Experimental suggestions	Annexin V-AbFluor developed by Abbkine ™ The 488 Cell Apoptosis Detection Kit contains Abbkine's proprietary AbFluor ™ Annexin V labeled with 488 green fluorescent dye can be used to identify and quantify apoptotic cells using flow cytometry or fluorescence microscopy

Product attributes

Kit components	Annexin V binding buffer (5x) • Annexin V-AbFluor ™ 488 • Propidium Iodide (PI)
Features&Advantages	• Abbkine AbFluor ™ 488 is a substitute for FITC, which not only has better fluorescence intensity and photostability, but is also not affected by pH • Annexin V-AbFluor ™ 488: Ex/Em = 491/517 nm; PI: Ex/Em = 535/617 nm (DNA dye) Suitable for flow cytometry and fluorescence microscopy
Save Suggestions	From the date of shipment, it can be stably stored at 4 ° C for 6 months. Please store in the dark and avoid freezing.
transport conditions	Blue Ice Transport
Warning	The products listed in this article are for research purposes only and are not suitable for human or clinical diagnosis. The applications recommended by our product are not intended to violate any license by using our product. We do not assume any responsibility for any infringement or other violations that may occur when using this product.

Additional information

background

Apoptosis is a fundamental biological phenomenon that refers to the autonomous and orderly cell death controlled by genes to maintain internal environmental stability. It plays a necessary role in the removal of unwanted or abnormal cells in multicellular organisms. In normal living cells, phosphatidylserine (PS) is located on the inner side of the cell membrane, while in the early stages of apoptosis, PS can flip from the inner side of the cell membrane to the surface of the cell membrane and be exposed to the extracellular environment. Annexin V is a calcium dependent phospholipid binding protein with a molecular weight of 35-36KDa, which can specifically bind to PS with high affinity. Early cell apoptosis can be easily and quickly detected by labeling Annexin V with fluorescein or biotin. Propidine iodide (PI) is a nucleic acid dye that cannot penetrate the intact cell membrane, while the cell membrane of early apoptotic cells is intact and resistant to PI. But in the late stage of apoptosis and dead cells, PI can penetrate the cell membrane and stain the nucleus. Therefore, by matching Annexin V with PI, it is possible to distinguish between cells in the early and late stages of apoptosis and dead cells. PI can be excited by 488532 or 546nm laser lines and emit red fluorescence.

alias

Annexin 5; Annexin V; AbFluor™ 488