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Shanghai Qiaoxin Trading Co., Ltd
Room 704, Building 13, No. 229 Ningguo Road, Yangpu District, Shanghai
SuperKine Enhanced Antibody Dilution Solution
| Product English Name | SuperKine™ Enhanced Antibody Dilution Buffer |
| Experimental suggestions | SuperKine ™ Enhanced antibody diluent provides an enhanced reagent that can be directly used to dilute primary or secondary antibodies in appropriate proportions with this product. |
| Kit components | Enhanced antibody diluent |
| Features&Advantages | SuperKine ™ Enhanced antibody diluent, based on the innovative optimization of classic antibody diluent components, improves the storage time of diluted antibodies and can be reused multiple times, combining quality and affinity price. |
| Precautions during use | Do not mix components from different batch numbers and manufacturers; Otherwise, it may lead to abnormal results. Incubate antibodies at 4 º C as much as possible to prolong the lifespan of diluted antibodies and facilitate repeated use. If non-specific bands interfere with the experiment, one can try to reduce the amount of protein loaded, decrease the concentration of primary and secondary antibodies, or shorten the exposure time appropriately. The product has undergone rigorous quality testing. Welcome to contact us anytime. We are committed to customer success and satisfaction. |
| Save Suggestions | From the date of shipment, it can be stably stored at 4-8 ° C for one year. Exposure to the sun or any other strong light may affect product quality, and short-term laboratory lighting will not have an impact. |
| transport conditions | Blue Ice Transport |
| Warning | The products listed in this article are for research purposes only and are not suitable for human or clinical diagnosis. The applications recommended by our product are not intended to violate any license by using our product. We do not assume any responsibility for any infringement or other violations that may occur when using this product. |
| background | SuperKine ™ Enhanced antibody diluent (SuperKine) ™ Enhanced Antibody Dilution Buffer is a ready to use, universal type suitable for diluting antibody dilutions in immune antibody assays (IF, IHC, ELISA, WB) experiments. This product can be used for dilution and preparation of primary or secondary antibodies. It contains enhancers, blocking agents, and stabilizers for antibody binding reactions to enhance immune responses, reduce non-specific binding, and achieve better results. It can be reused repeatedly to save valuable antibodies. This product does not contain preservatives such as protein, phosphate, sodium azide, or thimerosal, and is suitable for diluting most types of antibodies, including HRP and AP labeled antibodies. |
| alias | Enhanced Antibody Dilution; Antibody Dilution |

Fig.1.Superkine ™ Comparative WB experiments were conducted between the enhanced antibody dilution solution (BMU103-CN), the regular antibody dilution solution (TBST), and the same level competitor (B brand), showing that Superkine ™ The dilution solution has a higher signal-to-noise ratio. Sample: HEK293 cells; Primary antibody: Mouse anti NFkBp65 monoclonal antibody (ABM0017, 1:5000);

Fig.2. Left image: SuperKine ™ The results of IHC validation of enhanced diluent are shown in the figure on the right: negative control without added primary antibody. The sample is mouse liver tissue. Primary antibody: TNF - α polyclonal antibody (ABP0127, 1:200), secondary antibody: goat anti rabbit IgG (A21020, 1:300).

Fig.3. Left image: SuperKine ™ The results of the IF validation of the enhanced diluent are shown in the negative control without the addition of primary antibody in the figure on the right. The sample is Hela cells. The primary antibody is CK7 monoclonal antibody (ABM0018, 1:200), and the secondary antibody is Dylight 549, Goat Anti Mouse IgG (A23310, 1:200).

Fig.4. The image shows SuperKine ™ The results of ELISA validation using enhanced diluent, KET7011 EliKine after using this diluent ™ The standard curve of the mouse interleukin-12 p70 ELISA quantitative kit.