General Internal Reference Antibody Cocktail Set

NegotiableUpdate on 05/06

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Overview

KTD101-CN General Internal Reference Antibody Cocktail Set 798/Set $r $n • GAPDH Mouse Monoclonal Antibody (43KD) 60ul Original Price 498 $r $n • β - actin Mouse Monoclonal Antibody (37KD) 60ul Original Price 498 $r $n • β - Tubulin Mouse Monoclonal Antibody (55KD) 60ul Original Price 498 $r $n • Animal Cell Total Protein WB Positive Control 100ul Non sale $r $n • SuperKine Enhanced antibody diluent 10

Product Details

General Internal Reference Antibody Cocktail Set

Product Information

Product English Name	Universal Loading Control Antibody Cocktail
Experimental suggestions	General Internal Reference Antibody Cocktail SetProvide a comprehensive and detailed method for quickly and conveniently detecting the content of reference proteins in various samples.

Product attributes

Kit components	GAPDH mouse monoclonal antibody (2B5) β - actin mouse monoclonal antibody (1C7) β - Tubulin mouse monoclonal antibody (3G6) Animal cell total protein WB positive control • SuperKine ™ Enhanced antibody diluent
Features&Advantages	General Internal Reference Antibody Cocktail SetBased on the innovative optimization of the classic WB combination kit, it improves the storage time of diluted antibodies and can be reused multiple times, combining quality and affinity price.
Precautions during use	Do not mix components from different batch numbers and manufacturers; Otherwise, it may lead to abnormal results. If non-specific bands interfere with the experiment, one can try to reduce the amount of protein loaded, decrease the concentration of primary and secondary antibodies, or shorten the exposure time appropriately. The product has undergone rigorous quality testing. Welcome to contact us anytime. We are committed to customer success and satisfaction.
Save Suggestions	From the date of shipment, it can be stably stored at 4-8 ° C for one year. Exposure to the sun or any other strong light may affect product quality, and short-term laboratory lighting will not have an impact.
transport conditions	Blue Ice Transport
Warning	The products listed in this article are for research purposes only and are not suitable for human or clinical diagnosis. The applications recommended by our product are not intended to violate any license by using our product. We do not assume any responsibility for any infringement or other violations that may occur when using this product.

Additional information

background

The universal reference antibody cocktail is a set that includes antibodies commonly used in immunological experiments (WB, IHC-P, IF) such as β - actin, GAPDH, and β - Tubulin, as well as antibody dilutions. There are many types of reference antibodies, therefore, it is necessary to choose a suitable reference antibody, which should follow the following three principles: 1 Considering the species genus, for mammalian tissues and cells, commonly used reference proteins include β - actin, Tubulin, GAPDH, Lamin B1, PCNA, Na+/K+- ATPase, etc; For plant samples, commonly used are Plant actin and Rubisco. Consider the molecular weight of the target protein. 2. The selected reference protein should have a different molecular weight (>5 KDa) from the target protein in order to distinguish the target protein band. For high molecular weight proteins (over 100 kDa), we recommend customers to use Vinculin as an internal reference; 2. Consider the expression localization of the target protein and select corresponding internal parameters, such as whole cell, nucleus, mitochondria, cell membrane, etc

Images and explanations

Fig.1. β-Actin 表达的WB实验，样本分别为Rat brain (lane 1）， HeLa cell lysate (lane 2）， Mouse brain (lane 3）各10 ug，一抗为β-Actin小鼠单克隆抗体(1C7)（A01010，1：5000），二抗为山羊抗小鼠IgG（A21010， 1: 10000）。

Fig. 1. WB experiment on β - actin expression, samples were Rat brain (lane 1), HeLa cell lysis (lane 2), and Mouse brain (lane 3), each with 10 ug. The primary antibody was β - actin mouse monoclonal antibody (1C7) (A01010, 1:5000), and the secondary antibody was goat anti mouse IgG (A21010, 1:10000).

Fig.2. GAPDH表达的WB实验，样本分别为Hela (lane 1）， rat brain (lane 2）， Mouse brain (lane 5）各10 ug，一抗为GAPDH小鼠单克隆抗体(2B5)（A01020，1：10000），二抗为山羊抗小鼠IgG（A21010， 1: 10000）。

Fig.2. WB experiment on GAPDH expression, with samples of Hela (lane 1), rat brain (lane 2), and mouse brain (lane 5) each containing 10 ug. The primary antibody was GAPDH mouse monoclonal antibody (2B5) (A01020, 1:10000), and the secondary antibody was goat anti mouse IgG (A21010, 1:10000).

Fig.3. β-Tubulin表达的WB实验，样本分别为Rat brain (lane 1）， A549 cell(lane 2）， Mouse brain (lane 3）各10 ug，一抗为β-Tubulin小鼠单克隆抗体(2B5)（A01030，1：10000），二抗为山羊抗小鼠IgG（A21010， 1: 10000）。

Fig. 3. WB experiment for β - Tubulin expression, samples were Rat brain (lane 1), A549 cell (lane 2), and Mouse brain (lane 3), each with 10 ug. The primary antibody was β - Tubulin mouse monoclonal antibody (2B5) (A01030, 1:10000), and the secondary antibody was goat anti mouse IgG (A21010, 1:10000).