*Macrophage removal, Clophosome Clophosome

*Macrophage removal, Clophosome Clophosome

Product keywords:

Clophosphoome-A (anionic); Clodronate Liposomes; The macrophage 'suicide' approach involves the self destruction pathway of macrophages; Clophosphoome-M neutral chlorfenapyr; Clodronate disodium chlorophosphonate; CAS：22560-50-5；

Research background:

MacrophagesBelonging to ancient multifunctional cells, it plays an important role in the regulation of innate and acquired host defense systems, internal environmental homeostasis, and a large number of physiological activities. It is also the main tool used to engulf soluble mediators of intracellular digestion, production, and release, such as cytokines, chemokines, and nitric oxide (NO). Macrophages are intrinsic cells of all organs in the body that can be recruited to immune sites. The study of the functions of various macrophage subpopulations is often hindered by the lack of reliable and specific methods for in vivo clearance of macrophages. Therefore, it is extremely important to develop an effective macrophage 'suicide' approachLiposome mediated macrophage clearance pathway (i.e. Clophosphoome) ®）It has been widely recognized, not only in immunological research, but also in many other fields of biomedicine.

Mechanism of action:

Clophosome ®- A (Chlorfenapyr), a negatively charged chlorophosphonic acid liposomeIt is a generation of neutral Clophosome ®- The upgraded product of N belongs to the second generation of highly efficient in vivo macrophage depletion agents, which essentially consist of large single-cell liposomes (LUVs) encapsulating chlorophosphonic acid molecules. Combined with the characteristic that free chlorophosphonic acid is difficult to penetrate the phospholipid bilayer and cell membrane of liposomes, but liposomes can be easily engulfed by macrophages. After being engulfed by Clophosome, the phospholipid bilayer of liposomes is disrupted by lysosomal phospholipase action, allowing the water-soluble drug chlorophosphonic acid inside to be released into the cell. When chlorophosphonic acid accumulates in cells and reaches the threshold concentration at which it begins to exert its therapeutic effect, the cells are irreversibly damaged and induced to die through apoptosis. The free flowing chlorophosphonic acid released by macrophages after death has an extremely short half-life in the circulatory system and is rapidly cleared in the urinary system.[See Figure 1]

If liposomes can be fully injected into the body, macrophages from different sources of the body can be cleared, including liver (Kupffer cells, Kupffer cells), spleen (different subpopulations of macrophages), lungs (alveoli but not small intestine macrophages), peritoneal cavity, lymph nodes, joints (engulfing synovial lining cells), and testes.

Product Features

1. Product Name: Clophosomet-A Chlorfenapyr (Anionic)

2. Active ingredient: Clodronate disodium (CAS: 22560-50-5), 7mg/ml

3. Lipid composition: Phosphatidylglycerin, Phosphatidylglycerin, and Cholesterol

4. Body solution: physiological phosphate salt (20mM sodium phosphate, 0.9% NaCl), pH 7.0-75

5. Appearance color: Milk like, freely flowing liposome dispersion; There may be some liposome precipitation, which can be evenly dispersed after gentle mixing.

6. Storage and handling: Store at 2-8 ° C and do not freeze or thaw. Use the front room temperature to warm up and mix well.

7. Validity period: 6 months before opening.

Product advantages:

1. The formula has a high chloride phosphate/lipid ratio, a high encapsulation rate of>90%, and a high concentration of 7mg/ml;

2. Suitable for multiple administration routes, including intravenous, intraperitoneal, subcutaneous, nasal, tracheal, etc., single or multiple administration;

3. The upgraded formula uses negatively charged phosphatidylglycerol UV liposomes, which have better macrophage clearance (depletion) efficiency; For example, 0.2ml of clopidogrel was administered intravenously as a single dose, and after 24 hours, it was found that>90% of military cells derived from the spleen (red pulp macrophages) could be cleared.

4. The product has high stability and a shelf life of 6 months; Long term storage in a refrigerator (2-8 ° C) still maintains high activity, uniformity, and almost no precipitation;

5. The product is sterile, non-toxic, and odorless;

6. The product is non precipitating, non viscous, and easy to inject;

7. Simultaneously provide blank control liposome box and fluorescently labeled liposomes;

Application example:

Purification effect diagram of splenic macrophages

Product ordering:​For larger packaging or product technical issues, please call/consult online

There are currently two commonly used 1ml mini packs available in stock

Company Introduction:

The series of products Clophosome and Clophosome-A produced by Formumax Scientific are currently ideal sodium chlorophosphonate liposomes on the market for efficient macrophage depletion. This group of products has many advantages such as high activity, long shelf life, easy use, and sterility and non toxicity. The advantages of this dosage form include high concentration, high encapsulation efficiency, non viscosity, no precipitation, and easy injection. Especially the optimized formula ensures that this product can maintain its high activity and uniform sediment free state even after being stored in the refrigerator for several months. The 6-month product shelf life we offer is more than double that of similar products from other manufacturers. This group of products is suitable for multiple routes of administration, including intravenous injection, subcutaneous injection, tracheal administration, and direct injection. After a single intravenous or intraperitoneal injection of 0.2ml Clophosome, macrophage depletion in the spleen and liver can last for over 80% for a week starting from 24 hours. If injected every 4 days, efficient macrophage depletion can last for several weeks. Formumax Scientific's transformation product Clophosome-A not only maintains all the advantages of Clophosome, but also achieves a macrophage depletion rate of over 90% after a single injection. Highly popular among scientific research users. This group of products is a research tool for immunology, oncology, and other biological fields.

Formumax Scientific was founded in 2008 and is located in Silicon Valley, California, USA, the world's center of high technology and biotechnology
Formumax Scientific holds a high position in the field of nanoliposome drug delivery
Formumax Scientific has a world-class technical team with thirty years of experience in drug delivery research and development
Formumax Scientific is committed to providing high-quality services for the pharmaceutical and biotechnology industries
Formumax Scientific adheres to its reputation and quality assurance, with hundreds of loyal customers spread all over the world

*Macrophage removal, Clophosome ClophosomeThe above product introduction