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Shanghai Qiaoxin Trading Co., Ltd
Room 704, Building 13, No. 229 Ningguo Road, Yangpu District, Shanghai
| Product English Name | SuperKine™ West Femto Maximum Sensitivity Substrate |
| Experimental suggestions | SuperKine ™ The ultra sensitive ECL luminescent liquid (Feck grade) provides a highly sensitive chromogenic solution to enhance the chemical signal of immunological experiments. To obtain excellent experimental results, you need to optimize all your experimental elements, including sample quantity, antibody concentration, membrane and sealing agent usage. |
| Kit components | Reagent A and reagent B of equal volume |
| Features&Advantages | SuperKine developed by Abbkine ™ Ultra sensitive ECL luminescent liquid (Feck grade), based on the classic peroxidase substrate system innovation and optimization, is used to enhance chemiluminescence (ECL). It can directly replace other expensive ECL products without the need to re optimize experimental conditions, and is a combination of quality and affinity price. |
| Precautions during use | Do not mix components from different batch numbers and manufacturers; Otherwise, it may lead to abnormal results. To achieve excellent experimental results, you need to optimize all your experimental elements, including sample quantity, antibody concentration, membrane and sealing agent usage. Mix two substrate components in a 1:1 ratio to prepare a substrate working solution. Please pay attention to replacing the nozzle during the suction process of liquid A and liquid B. Suggest corresponding 0.1-0.2 mL SuperKine for every 1 cm2 membrane size ™ Enhanced ECL luminescent liquid (Pick level) working fluid. |
| Save Suggestions | From the date of shipment, it can be stably stored at 4-8 ° C for one year. Exposure to the sun or any other strong light may affect product quality, and short-term laboratory lighting will not have an impact. |
| transport conditions | Blue Ice Transport |
| Warning | The products listed in this article are for research purposes only and are not suitable for human or clinical diagnosis. The applications recommended by our product are not intended to violate any license by using our product. We do not assume any responsibility for any infringement or other violations that may occur when using this product. |
| background | After years of development, chemiluminescence technology has developed into various luminescent systems, but the technology systems commonly used in laboratories are still based on luminol or its derivatives (such as isoluminol). The Western fluorescence detection reagent ECL immunoblotting chemiluminescence solution is an experimental auxiliary reagent designed to detect proteins fixed on solid phase membranes (such as NC, PVDF, etc.) with a size of 30 to 50 picograms or more through a non radioactive (horseradish peroxidase) luminescence system, and record their immunoblotting by X-ray film (radiographic film). |
| alias | Enhanced Chemiluminescence; ECL; Chemiluminescence; Electrochemiluminescence |

Fig. Figure: The sample is a recombinant protein TNF - α protein (PRP1113, 17KD), the primary antibody is TNF - α Polyclonal Antibody (ABP0127, 1:2500), the secondary antibody is HRP, Goal Anti rabbit IgG (A21020, 1:10000), and the exposure time is 30 seconds.