Succinic acid producing actinomycetes

NegotiableUpdate on 05/06

Model

Nature of the Manufacturer: Producers

Product Category

Place of Origin

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Overview

Succinic acid producing actinomycetes, inclined surfaces, puncture bacteria, and freeze-dried powder should be stored at 4-10 degrees Celsius, while glycerol producing bacteria should be stored at -80 degrees Celsius. Research; Teaching; classification Analysis and testing.

Product Details

Strain Name: Actinobacterium Succinate Producing

English name:Actinobacillus succinogenes‍

Culture medium tryptone: 17.0, soy peptone: 3.0, sodium chloride: 5.0, dipotassium dihydrogen phosphate: 2.5, glucose: 2.5g, pH: 7.3 ± 0.2 (25 ℃)

Growth conditions at 30 ℃; 18-24h; aerobic

Storage conditions 2-8 ℃

Security Level 1

The morphology of the colony is 0.5-1mm in diameter, circular, with neat edges, opaque, yellow on the front, light in color, raised in the middle, bright on the surface, smooth on the surface, moist in texture, easy to pick up, G - (red), rod-shaped, purity: pure

模式菌株 yes

Step 1: Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow.

Notes:

1. Microbial strains should be stored in a low temperature, clean, and dry place. Prolonged storage at room temperature can cause strain decay;

2.Succinic acid producing actinomycetesThe operation should be carried out under sterile conditions, and after inoculation, it should be sterilized before disposal;

3. The storage time for inclined and puncture strains is usually 3-6 months, and they should be transferred in a timely manner according to the condition of the strains; The storage time of freeze-dried powder is usually 2-25 years; The storage time of glycerol bacteria is 2-5 years.