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Room 11545, Building 14, No. 3265 Jinhai Road, Fengxian District, Shanghai
Shanghai Xuanya Biotechnology Co., Ltd
Room 11545, Building 14, No. 3265 Jinhai Road, Fengxian District, Shanghai
Strain name: Hemophilus parahaemolyticus
English name:Haemophilus parahaemolyticus Pittman
Cultivate multi valent peptone: 18.0, starch: 1.0, sodium chloride: 5.0, agar: 15.0, fibroblast free sheep blood: 40mL, pH: 7.4 ± 0.2 (25 ℃)
Growth conditions at 37 ℃; 24-48h;5%CO2;
Storage conditions 2-8 ℃
Security Level 2
The morphology of the colony is 1-2mm in diameter, circular, with neat edges, opaque, gray white on the front, raised in the middle, smooth and bright on the surface, moist texture, easy to pick up, G - (red), Bacillus, purity: pure
Separation of substrates for chronic pulmonary infection
模式菌株 yes
Step 1: Prepare 1-2 fresh tablets as mentioned above; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water into the freeze-drying tube, dissolve it thoroughly, and mix well; ④ Transfer 0.2mL of bacterial suspension into a plate, apply evenly, and repeat twice to obtain two plates; ⑤ Cultivate under the above conditions, and the strain can be used once it grows.
Notes:
1. Microbial strains should be stored in a low temperature, clean, and dry place. Prolonged storage at room temperature can cause strain decay;
2.Hemophilus parahaemolyticusThe operation should be carried out under sterile conditions, and after inoculation, it should be sterilized before disposal;
3. The storage time for inclined and puncture strains is usually 3-6 months, and they should be transferred in a timely manner according to the condition of the strains; The storage time of freeze-dried powder is usually 2-25 years; The storage time of glycerol bacteria is 2-5 years.
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