PiChia fermentans

Strain name: Fermented Pichia pastoris

English name:Pichia fermentans‍

Malt extract agar (MA): 20.0g malt extract, 20.0g glucose, 1.0g peptone, 20.0g agar, 1.0L distilled water, natural pH. Sterilize at 121 ℃ for 15 minutes.

Growth conditions at 37 ℃; 3-4 days; Aerobic

Storage conditions 2-8 ℃

Security Level 1

The morphology of the colony is 1-2mm in diameter, circular, with neat edges, opaque, milky white on the front, raised in the middle, smooth and bright on the surface, moist texture, easy to pick up, G+(blue purple), cocci, purity: pure

Separate the base material KEFIR

Model strain no

Step 1: Prepare the two tablets mentioned above; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into 2 plates at a rate of 200 μ L per plate and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.

Notes:

1. Microbial strains should be stored in a low temperature, clean, and dry place. Prolonged storage at room temperature can cause strain decay;

2.PiChia fermentansThe operation should be carried out under sterile conditions, and after inoculation, it should be sterilized before disposal;

3. The storage time for inclined and puncture strains is usually 3-6 months, and they should be transferred in a timely manner according to the condition of the strains; The storage time of freeze-dried powder is usually 2-25 years; The storage time of glycerol bacteria is 2-5 years.

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