Seahorse XF Flex Real time Cell Metabolism Analyzer

AgilentSeahorse XF Flex Real time Cell Metabolism AnalyzerOxygen consumption rate (OCR), proton release rate (PER), extracellular acidification rate (ECAR), and ATP production rate can be measured in a 24 well plate, and the obtained detection results can be reported. This instrument provides optimized workflow and specialized consumables, enabling real-time metabolic analysis of 3D research models such as organoids and tissue materials. By using specialized consumables, a wider signal dynamic range can be provided.

OCR, PER, and ATP production rate are key attributes of energy metabolism and important measures of mitochondrial health, toxicity, glycolysis, and overall cellular function/dysfunction. Analyzing these parameters in more complex three-dimensional structures can provide deeper insights and enhance the transferability of research.

The XF Flex analyzer is also equipped with advanced software and advanced data analysis features provided by Agilent Seahorse Analytics software. These features greatly simplify the entire XF detection process from detection design to data QC and interpretation.

AgilentSeahorse XF Flex Real time Cell Metabolism AnalyzerofFeatures:

Flexible 24 well system for real-time metabolic analysis of two-dimensional (2D) and three-dimensional (3D) research models, with higher sensitivity and stability

Each detection hole will report multiple parameters, including OCR, PER or ECAR, and ATP generation rate

Tailored workflows and specialized consumables for organoid and tissue materials

A wider detection range, very suitable for research models on strong respiratory effects

A four drug dispensing system with automatic mixing function, capable of real-time evaluation of the immediate response of cells, tissue organs to substrates, inhibitors, and other compounds

Verified instrument performance ensures consistency of data within and between boards

A wider working environment temperature (4 ° C to 30 ° C) allows the analyzer to maintain an internal detection temperature of 16 ° C to 42 ° C

Intuitive user guided software, easy to create detection solutions

The automatic data quality check function in data analysis software will mark abnormal detection situations

The data file is compatible with advanced data analysis features in Seahorse Analytics, a reliable web-based data analysis tool

Performance indicators:

Technology:

Real time automatic energy metabolism measurement

The Agilent Seahorse XF analyzer can study key cellular functions such as mitochondrial respiration and glycolysis by measuring the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in porous plates. The Seahorse XF analyzer can perform real-time compound addition and mixing, label free analyte detection, and automatic measurement of OCR and ECAR.

Working Principle:

Real time monitoring of living cell bioenergy metabolism in microplates

The two main energy production pathways, mitochondrial respiration and glycolysis, involve cellular oxygen consumption and proton release rate, respectively. Seahorse XF technology uses label free sensors to detect extracellular changes in these analytes, in order to determine cellular respiration rate, glycolysis, and ATP production. This technology can be used to study metabolic changes in three-dimensional materials such as cultured cells, suspended cells, tissues, organoids, and/or multicellular clusters.

Create microchambers and calculate the extracellular flow rate per minute

The instrument lowers the probe plate into the detection hole. The sensor is located at a specific distance above the bottom of the hole, forming a very small transient microcavity, which is crucial for achieving high-sensitivity detection of Seahorse XF technology. As the oxygen and pH levels change, the instrument can record the corresponding changes in the sensor. The measurement time is usually 3 minutes and the rate calculation is automatically performed. After each measurement, raise the probe to restore the extracellular culture medium to its baseline conditions.

Inject up to 4 compounds for real-time testing of response or study of biological mechanisms

The probe board is also equipped with dosing ports (4 per well), which can inject regulatory factors into the cell wells during the analysis process. After completing the instrument configuration, the system will inject compound "A" into the analysis well and slowly mix to ensure uniform distribution of the compound in the analysis medium. All holes are processed synchronously in this way. The system will automatically execute any additional dosing and rate calculations specified in the subsequent measurement cycle and plan.

Application:

Explore the powerful functions of cellular metabolism

The Agilent Seahorse XF platform can measure in real-time the two main metabolic pathways of living cells (mitochondrial respiration and glycolysis), providing functional kinetic measurements of cellular bioenergy metabolism. Understanding how bioenergy parameters provide valuable information and serve as indicators for disease models, key cellular processes, and therapy discovery.

Immunometabolism

The immune cell processes, including activation, proliferation, and memory cell development, are driven by metabolic reprogramming, which can be regulated to enhance performance and control immune cell outcomes. Through functional real-time metabolic measurement, understand immune cell processes such as activation, proliferation, and memory cell development.

Cancer metabolism

Metabolism is a key driving factor for the growth of cancer malignant tumor cells. In order to transition towards a glycolytic phenotype overall, cancer cell proliferation usually needs to be upregulated or "metabolized" to accelerate energy demand and generate structural units, ultimately promoting cancer cell growth. By conducting real-time functional bioenergy metabolism analysis on living cells, we can reveal the metabolic characteristics of cancer and gain a deeper understanding of cancer cell biology.