Rat transforming growth factor beta (TGF - β) ELISA kit

Attention: The products sold by our companyRat transforming growth factor beta (TGF - β) ELISA kitSigma Proxy VIII Ag reagent kitIt is only used for scientific research experiments and cannot be used for food or clinical use, edible drugs, etc.

While ensuring quality, the company,Rat transforming growth factor beta (TGF - β) ELISA kitSigma Proxy Rat TGF - β ELISA KitWith the aim and concept of competitive prices, fast delivery, thoughtful service, and honest transactions, we are committed to various biological reagents, antibodiesELISASales promotion and service work for reagent kits, cytokines, and consumables. The company provides free testing services to better serve you. For details, please consult or consult online personnel.Imported original reagent kits require a delivery time of two to three weeks. If you need to place an order,You can consult or place an order through the following methods, and we sincerely hope to cooperate with you:

1.Can you comeDian consulted and ordered directly from us. (We will ship to you within three working days upon receipt of payment)

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Sample processing and requirements：

1. Serum: Natural coagulation of blood at room temperature10-20Minutes, centrifuge20About minutes（2000-3000turn/Divided). Carefully collect the supernatant. If precipitation occurs during storage, centrifuge again.

2. Plasma: should be selected according to the requirements of the specimenEDTAOr sodium citrate as an anticoagulant, mixed10-20After minutes, centrifuge20About minutes（2000-3000turn/Divided). Carefully collect the supernatant. If sediment forms during storage, it should be centrifuged again.

3. Urine: Collect with sterile tube, centrifuge20About minutes（2000-3000turn/Divided). Carefully collect the supernatant. If sediment forms during storage, centrifuge again. Refer to the implementation for pleural effusion, ascites, and cerebrospinal fluid.

4. Cell culture supernatant: When detecting secreted components, collect them using sterile tubes. centrifugation20About minutes（2000-3000turn/Divided). Carefully collect the supernatant. When detecting the components inside cells, usePBS（PH7.2-7.4）Dilute the cell suspension until the cell concentration reaches100ten thousand/mlabout. By repeatedly freezing and thawing, cells are destroyed and intracellular components are released. centrifugation20About minutes（2000-3000turn/Divided). Carefully collect the supernatant. If precipitation forms during storage, it should be centrifuged again.

5. Tissue specimen: After cutting the specimen, weigh it. Add a certain amountPBS，PH7.4Quickly freeze and store in liquid nitrogen for future use. The specimen remains intact even after melting2-8℃The temperature. Add a certain amountPBS（PH7.4）Use manual or homogenizer to homogenize the specimen thoroughly. centrifugation20About minutes（2000-3000turn/Divided). Carefully collect the supernatant. After packaging, one portion is to be tested, and the rest is to be frozen for future use.

6. Extract the specimen as soon as possible after collection, according to relevant literature, and conduct experiments as soon as possible after extraction. If the experiment cannot be conducted immediately, the specimen can be placed in-20℃Save, but avoid repeated freeze-thaw cycles.

7. Cannot detect containingNaN3The sample, becauseNaN3Inhibition of horseradish peroxidase（HRP）Activity.

The company is ensuring qualityRat transforming growth factor beta (TGF - β) ELISA kitSigma Proxy VIII Ag reagent kitAt the same time, with the aim and concept of excellent price, fast delivery, thoughtful service, and honest transactions, we are committed to various biological reagents, antibodiesELISASales promotion and service work for reagent kits, cytokines, and consumables. The company provides free testing services to better serve you. For more details, please consult Dian or consult our online staff.