Mouse platelet activating factor ELISA kit

Product Code: WTA4614, Product Name: Imported Mouse Platelet Activating Factor (PAF) ELISA Kit, English Name:mouse platelet activating factor,PAF ELISA Kit， Brand: Imported original/Domestic
Attention: The products sold by our companymousePlatelet activating factor ELISA kit imported AT-III kitOnly used for scientific research experiments, no, the human thrombopoietin ELISA kit can be used for food or clinical use, edible drugs, etc.
Shanghai Weiting Biotechnology Co., Ltd. has long provided ELISA kits, mouse and rat ELISA kits, human ELISA kits, rabbit ELISA kits, bovine ELISA kits, standard samples, control samples, biological reagents, scientific research antibodies, cell lines, biological consumables, etc. We also act as agents for various foreign companies' products such as Sigma, R&D, ScienCell, ATCC, Wak0, Omega, Worthington, MBI, Bioworld, Calendon, etc. Affordable price, guaranteed quality, and excellent reputation. Please inquire and request ELISA reagent kits and product manuals. For details of the imported AT-III kit for human platelet activating factor ELISA, please contact our online customer service! We will contact you as soon as possible!

ShanghaiWei TingBiotechnology Co., Ltd. provides long-term servicesELISAtest kitELISADetection kit, immunohistochemistry kit, humanElisaReagent kit, rats and miceElisaReagent kits, antibody antigens, affordable prices,companyI acted as an agentsigma,R&D,Hycult，Abnova，Abcam，BD，ScienCell,ATCC, Waiting for products from various foreign companies. Affordable prices, guaranteed quality, and top reputation.Imported original reagent kits require a delivery time of two to three weeks. If you need to place an order,PleasecomedianConsult or consult online personnel.
To place an order, please click on the button in the upper left cornerOnline Customer ServiceFor consultation, we will contact you as soon as possible!

ELISAReagent kit instructions：

1Detection principle: using double antibody sandwichABC-ELISAlaw

2There may be discrepancies between the Chinese and English manuals, please refer to the English manual for accuracy.

3The newly opened enzyme-linked immunosorbent assay (ELISA) wells may contain a small amount of aqueous substances, which is a normal phenomenon and will not have any impact on the experimental results.

4Storage of reagent kit:-20℃(When not in use for a long time);2-8℃(When frequently used).

5Salt precipitation may occur when storing concentrated detergent at low temperatures. When diluting, it can be dissolved by heating it in a water bath.

thatElisaThe test kit can detect samples such as blood samples, tissue fluid samples, urine samples, fecal samples, cerebrospinal fluid samples, pleural and peritoneal fluid samples, etc. (Please consult for specific details)

ElisaOperation steps of the reagent kit (specific operation shall be subject to the instructions):

Before starting the experiment, please prepare all reagents in advance. When diluting reagents or samples, they should be mixed thoroughly and avoid foaming as much as possible. A standard curve should be performed for each test. If the sample concentration is too high, dilute it with sample diluent to make the sample meet the detection range of the kit.

1. Sample addition: set blank holes, standard holes, and test sample holes respectively. Add sample diluent to blank hole100MlAdd standard samples or test samples separately to the remaining holes100MlBe careful not to have bubbles. Add the sample to the bottom of the enzyme-linked immunosorbent assay (ELISA) plate well, avoiding touching the well wall as much as possible. Gently shake and mix well. Cover the ELISA plate with a lid or film,37℃reaction120minute

To ensure the validity of the experimental results, please use a new standard solution for each experiment.

2. Discard the liquid, shake dry, no need to wash. Add biotin labeled antibody working solution to each well100Ml（取1MlBiotin labeled antibody addition99MlThe ratio of biotin labeled antibody dilution solution should be prepared, gently mixed, and prepared within one hour before use,37℃, sixtyminute

3. Nurture and nurture60After minutes, discard the liquid in the hole, shake dry, and wash the plate3Next time, soak each time1-2minute,350Ml/Each hole, shake dry.

4. Add horseradish peroxidase labeled avidin working solution (same as biotin labeled antibody working solution) to each well100Ml，37℃，60minute

5. Nurture and nurture60After minutes, discard the liquid in the hole, shake dry, and wash the plate5Next time, soak each time1-2minute,350Ml/Each hole, shake dry.

6. Add substrate solution to each well in sequence90Ml，37℃Avoid light and display colors（30Within minutes, the front of the standard sample is visible to the naked eye3-4The hole has a clear gradient in blue, followed by3-4If the pore gradient is not obvious, it can be terminated.

7. Add termination solution to each well in sequence50MlTerminate the reaction (blue immediately turns yellow). The order of adding termination solution should be as similar as possible to the order of adding substrate solution. To ensure the accuracy of the experimental results, the termination solution should be added as soon as the substrate reaction time is up.

8. Using an enzyme-linked immunosorbent assay (ELISA)450nmMeasure the optical density of each hole in order of wavelength（ODValue). After adding termination solution15Testing within minutes.

Note:

1. When using the reagent kit for the first time, users should centrifuge various reagent tubes for several minutes to concentrate the reagents at the bottom of the tubes.

2. Leave one well as a blank zeroing well for each experiment, without adding any reagents, only adding substrate solution afterwards2N H2SO4When measuring, first use this hole to adjustODValue to zero.

3. To prevent sample evaporation, the reaction plate is placed in a sealed box covered with a damp cloth during the experiment, and the enzyme-linked immunosorbent assay plate is covered or coated with a film.

4. For unused enzyme-linked immunosorbent assay (ELISA) plates or reagents, please contact2-8℃preservation. Standard samples, biotin labeled antibody working solution, and horseradish peroxidase labeled avidin working solution should be prepared and used according to the required amount. Do not reuse diluted standards, biotin labeled antibody working solution, or horseradish peroxidase labeled avidin working solution.

5. It is recommended to set up dual well testing when testing samples to ensure the accuracy of the test results.

ElisaTest kit detection range (pleasecomedianConsultation and Request

Consultation and orderingmousePlatelet activating factor ELISA kit imported AT-III kitThe method:
1. You can directly consult and place an order with us.
2. You can click on this page for online personnel consultation and ordering.
3. You can leave a message in the message bar at the bottom of the page, and we will contact you within half a working day.
4. You can inquire and place an order through emailMouse platelet activating factor ELISA kit imported AT-III kit.