Human platelet-derived growth factor ELISA kit

Product Code: WTA4613, Product Name: Human platelet-derived growth factor (PDGF) ELISA Kit Double Antibody Method, English Name: Human platelet-derived growth factor, PDGF ELISA Kit, Brand: Imported original/Domestic
Attention: The reagents sold by our company,Human platelet-derived growth factor ELISA kit dual antibody MHC Ⅰ/HLA Ⅰ kitOnly used for scientific research experiments, not for food or clinical use, edible drugs, etc.

Shanghai Weiting Biotechnology Co., Ltd. is one of the main suppliers in China. The company acts as an agent for ELISA kits of different price levels from original R&D and BD brands in the United States. We also act as agents for various foreign companies' products such as Sigma, R&D, ScienCell, ATCC, Wak0, Omega, Worthington, MBI, Bioworld, Calendon, etc., including standard samples, reference materials, biological reagents, scientific research antibodies, cell lines, and biological consumables. Affordable prices and free testing services are also provided.Human platelet-derived growth factor ELISA kit dual antibody MHC Ⅰ/HLA Ⅰ kitPlease communicate with our online customer service for details!

Weiting Biotechnology Co., LtdI acted as an agentsigma,R&D,Hycult，Abnova，Abcam，BD，ScienCell,ATCC, Waiting for products from various foreign companies. Affordable prices, guaranteed quality, and top reputation.Imported original reagent kits require a delivery time of two to three weeks. If you need to place an order,PleasecomedianConsult or consult online personnel.this companyHuman platelet-derived growth factor ELISA kit dual antibody MHC Ⅰ/HLA Ⅰ kitThe reagent kit is for research purposes only and should not be used in clinical or human experiments. Any consequences arising from this shall be borne by the experimenter, and our company shall not be held responsible.

ELISAComposition of reagent kit and preparation of reagents

1. Enzyme linked immunosorbent assay (ELISA) plate: one piece（96Hole)

2. Standard product (freeze-dried product):2Each bottle should be diluted with sample diluent before use1mlAfter covering, let it sit still10More than minutes, then repeatedly invert/Rub to aid dissolution, with a concentration of1,600 pg/mlDilute it to400 pg/mlAfterwards, perform a series of dilutions (note: do not directly perform dilutions in the plate) and dilute them separately to400 pg/ml，200 pg/ml，100 pg/ml，50 pg/ml，25 pg/ml，12.5 pg/ml，6.25 pg/mlThe sample diluent is directly used as the standard concentration0 pg/mlBefore use15Prepare within minutes. As configured200 pg/mlStandard product: Take0.5ml(Not less than)0.5ml）400 pg/mlThe above standard products contain0.5mlSample diluentEppendorfIn the tube, mix well, and so on for other concentrations.

3. Sample diluent:1×20ml.

4. Testing diluentA：1×10ml.

5. Testing diluentB：1×10ml.

6. detection solutionA：1×120Ml（1:100）Before use, test the diluentA 1:100Dilute, prepare according to the pre calculated total amount required for each experiment before dilution（100Ml/During actual preparation, more holes should be prepared0.1-0.2mlLike10Mldetection solutionAplus990MlTesting diluentAMix gently and prepare within one hour before use.

7. detection solutionB：1×120Ml/Bottle（1:100）Before use, test the diluentB 1:100dilute The dilution method is the same as the detection solutionA.

8. Substrate solution:1×10ml/Bottle.

9. Concentrated detergent:1×30ml/Bottles, dilute each bottle with distilled water during use25Twice.

10. Termination solution:1×10ml/Bottle（2N H2SO4）.

11. Film coating:5Zhang

12. an instruction manual:1copy

ELISAReagent kit self provided items

1. ELISA reader (it is recommended to refer to the instrument instructions for preheating in advance)

2. Micro liquid dispenser and suction head,EPpipe

3. Distilled water or deionized water, brand new filter paper

Collection and preservation of specimens

1. Serum:Please keep the whole blood sample at room temperature2Hour or4℃After overnight stay1000 x gcentrifugation20Minutes, take the supernatant for detection, or place the specimen in-20℃or-80℃Save, but avoid repeated freezing and thawing.

2. Plasma:availableEDTAOr heparin as an anticoagulant, after specimen collection30Within minutes2 - 8°C 1000 x gcentrifugation15Minutes, or placing the specimen in-20℃or-80℃Save, but avoid repeated freezing and thawing.

3. Other biological specimens:Please1000 x gcentrifugation20Minutes, take the supernatant for detection, or place the specimen in-20℃or-80℃Save, but avoid repeated freezing and thawing.

4. Sample processing:Recommended dilution for serum or plasma samples5,000Twice.

Note: Place above the mark4℃Save should be less than1week-20℃or-80℃All should be sealed and stored,-20℃Should not exceed1A month,-80℃Should not exceed2Months; Hemolysis of specimens can affect the results of subsequent tests, therefore hemolytic specimens are not suitable for this test.

Human platelet-derived growth factor ELISA kit dual antibody MHC Ⅰ/HLA Ⅰ kitOperation steps and detection range (pleasecomedianConsultation and Request