Merck Sigma Reagent Sigma Standard Supplier

Sigma standard websiteMerck Sigma Reagent Sigma Standard Supplier，Brand: Imported original/Domestic

Attention: The products sold by our companySigma standard websiteMerck Sigma Reagent Sigma Standard Supplier​ It is only used for scientific research experiments and cannot be used for food or clinical use, edible drugs, etc.

Shanghai Yixing Biotechnology Co., Ltd. is one of the major suppliers in China, acting as an agent for ELISA kits of different price levels from original R&D and BD brands in the United States. We also act as agents for various foreign companies' products such as Sigma, R&D, ScienCell, ATCC, Wak0, Omega, Worthington, MBI, Bioworld, Calendon, etc., including standard samples, reference materials, biological reagents, scientific research antibodies, cell lines, and biological consumables. Affordable prices and free testing services are also provided. For details, please place an order if neededSigma Standard Network Merck Sigma Reagent Sigma Standard Supplier Please consult the online customer service representative.

1.　sigmaELISAThe principle of
　　ELISAThe basis is the solid-phase immobilization of antigens or antibodies and enzyme labeling of antigens or antibodies. The antigen or antibody bound to the surface of the solid-phase carrier still maintains its immunological activity, while the enzyme labeled antigen or antibody retains both its immunological activity and enzyme activity. During the measurement, the tested specimen (the antibody or antigen being measured) reacts with the antigen or antibody on the surface of the solid-phase carrier. Separate the antigen antibody complex formed on the solid-phase carrier from other substances in the liquid by washing. Adding enzyme labeled antigens or antibodies also binds to the solid-phase carrier through reaction. At this point, the amount of enzyme on the solid phase is proportional to the amount of the test substance in the specimen. After adding the substrate to the enzyme reaction, the substrate is catalyzed by the enzyme to become a colored product, and the amount of the product is directly related to the amount of the analyte in the specimen. Therefore, qualitative or quantitative analysis can be performed based on the depth of the color. Due to the high catalytic efficiency of enzymes, they indirectly amplify the results of immune reactions, making the measurement method highly sensitive.

2.　ELISAType of
　　ELISAIt can be used to detect antigens or antibodies. There are three necessary reagents in this measurement method:

（1）Solid phase antibiotic antigen or antibody, i.e'Immunoadsorbent'（immunosorbent）； （2）Enzyme labeled antigens or antibodies, known as'complex'（conjugate）； （3）The substrate of enzyme reaction. Various types of detection methods can be designed based on the source of reagents, the condition of specimens, and the specific conditions of detection.

Consultation and ordering methods for this reagent:
1.CancomedianOrder directly from us.
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