Rat GRO/CINC-3 detection kit

ratGRO/CINC-3Test kitEnzyme linked immunosorbent assaylaw）

【Product Name】

Common name:ratGRO/CINC-3Detection kit (enzyme-linked immunosorbent assay)law）

English name:Rat GRO/CINC-3 Assay Kit

【specification】

96T

【intended use】

This kit can be used for quantitative detectionbigMouse serumEDTA-In plasma and cell supernatantGRO/CINC-3Restructuring and Natural FormsGRO/CINC-3All can be detected. This product is only used for research and not for diagnosis.

[Introduction]

Cytokine induced neutrophil chemoattractant3（CINC-3）Also known as ratsMIP- 2It is a chemokinealpha（CXC）Members of the subfamily are expressed in rat alveolar macrophages and fibroblasts stimulated by cytokines. ratCINC3The amino acid sequence and miceMIP-2The homology is88%.CINC3It is also considered an effective chemotherapy strategy factor for rat neutrophils. Nakagawa's team at Toyama Pharmaceutical Universityin1994Assuming that there are at least two types of neutrophils in ratsCINCReceptors, one type isCINC-3Specific receptors, the other being allCINCThe second shared receptor.

【Detection Principle】

This kit is based on the principle of solid-phase sandwich method and uses two highly specific antibodies for detection. Tetramethylbenzidine（TMB）As a chromogenic reagent, the color intensity is related tobigmouseGRO/CINC-3The quantity is directly proportional.

【Kit components】

1）Microplate: 96Kong, coated anti ratGRO/CINC-3rabbitIgGAffinity purification

2）Labeling antibodies: 30Double concentration,1x0.4ml，HRP-anti-ratGRO/CINC-3rabbitIgG Fab’Affinity purification

3）Standard product: 2x0.5mlRecombinant ratsGRO/CINC-3

4）EIABuffer solution: 1x30ml

5）Labeling antibody solution: 1x12ml

6）Substrate solution: 1x15ml

7）Termination solution: 1x12ml

8）Wash buffer concentrate: 1x50ml，40Double concentration