LP lipoprotein alpha detection kit (lipid metabolism)

LP lipoprotein alpha detection kit (lipid metabolism) is aEnzyme linked immunosorbent assay kit, imported agent of Kerunda Biotech

English name: Lipoprotein (a) ELISA

[Specification]

96T

This kit is used for quantitative detection of lipoproteins in human serum and plasma(a).

[Introduction]

Apolipoprotein（a），Apo（a）It is connected to through disulfide bondsLp（a）Apolipoproteins in particlesBThe glycoprotein.Apo（a）Formed by three different structural domains. One of the domains (referred to askringle 4, type2）There are multiple copies, each with a different quantity and determined by genetics, resulting in different sizes ofApo（a）. According to the method used, six types have been identified23Different speciesApo（a）Subtypes, ranging from300to900 kD.

Most individuals have two typesApo（a）Subtypes, although in some subjectsSDS-When Western blot analysis was performed after gel electrophoresis, no detection was foundApo（a）Take it.

Recently, due to numerous pieces of evidence indicating that circulating levels represent an independent risk factor for coronary heart disease, people are concerned aboutLp（a）The attention is very high.Lp（a）The level has been found to be a genetic risk factor for ischemic heart disease. High levels have been demonstrated in familial hypercholesterolemiaLp（a）The level of measurement may have clinical significance for predicting the risk of these patients.

The results are also availableLp（a）Published online, it can serve as a powerful indicator of cerebrovascular disease.Apo（a）Homologous with plasminogen activator.Lp（a）Inhibiting plasminogen activation, recent studies have shown that,Apo（a）Compete with plasminogen and bind to plasminogen receptors.Apo（a）These characteristics can explain the highLp（a）The relationship between concentration and myocardial infarction.

【 Detection Principle 】

This kit is a solid-phase two-point enzyme immunoassay. It is based on direct sandwich technology, in which two monoclonal antibodies are used toApo（a）Individual antigenic determinants on a molecule. During the incubation process, the sample containsApo（a）Antibody bound to peroxidaseApo（a）Antibodies and antibodies that bind to microporesApo（a）Antibodies react. A simple washing step can remove unbound enzyme-linked antibodies. through and with3,3'5,5'-Tetramethylbenzidine reaction is used to detect bound complexes. By adding acid to terminate the reaction, the colorimetric endpoint is obtained, which is read by spectrophotometry.

【 Kit Composition 】

eachLP lipoprotein alpha detection kit (lipid metabolism)All include for96The reagent for the well is sufficient for use43A sample and two sets of standard curves. For larger series of measurements, please use the combined reagents in packaging with the same batch number. The expiration date of the complete reagent kit is indicated on the external label. The recommended storage temperature is2–8℃。

Collection and processing of specimens

serum

Collect blood through venipuncture, coagulate it, and separate serum by centrifugation. The specimen can be found at2-8Store at ℃1week For longer periods of time, store the samples in-20At ℃. Avoid repeated freezing and thawing.

plasma

Collect blood through venipuncture until it containsEDTAOr heparin is used as an anticoagulant in a test tube, and the plasma portion is separated by centrifugation. The specimen can be found at2-8Store at ℃1week For storing samples for a longer period of time-20At ℃. Avoid repeated freezing and thawing.

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