Rat GRO (CINC-2 β) detection kit

Rat GRO (CINC-2 β) detection kit

Rat assay kit, imported cytokine assay kit

【Detection Principle】

Rat GRO (CINC-2 β) detection kitBased on the principle of solid-phase sandwich method,The microplate is pre coated with the first antibody, and the sample and standard are added to the microplate for the first reaction. Then join inHRPThe labeled second antibody undergoes a second reaction, and after washing to remove all unbound second antibodies, a color reaction occurs when the substrate solution is added.

【Kit components】

1）Microplate: 96Kong, coated anti ratGRO/CINC-2β RabbitIgGAffinity purification

2）Labeling antibodies: 1x10.5ml，HRP-anti-ratGRO/CINC-2β RabbitIgG Fab’Affinity purification

3）Standard product: 1x0.5mlRecombinant ratsGRO/CINC-2B

4）EIABuffer solution: 1x30ml

5）Labeling antibody solution: 1x12ml

6）Substrate solution: 1x15ml

7）Termination solution: 1x12ml

8）Wash buffer concentrate: 1x50ml，40Double concentration

【store】

Rat GRO (CINC-2 β) detection kitShould be stored in2-8At ℃, the expiration date should be indicated on the reagent kit, and expired reagents should not be used.

【detection steps】

Before useRat GRO (CINC-2 β) detection kitBalance to approximately room temperature30minute

（1）Determine sample blank wells, sample wells, and standard wells

（2）Translate into English100ML EIAAdd buffer solution to the blank well of the sample

（3）Translate into English100MLAdd samples and standards into the corresponding holes

（4）Sealing board, in37Incubate at ℃1hour

（5）Wash the microporous plate4The washing buffer added each time exceeds350ML

（6）Translate into English100MLAdd labeled antibodies to all wells, except for reagent blank wells

（7）Sealing board, in37Incubate at ℃30minute

（8）Wash the microporous plate5The washing buffer added each time exceeds350ML

（9）Translate into English100MLAdd substrate solution into each micropore

（10）Incubate at room temperature in the dark30minute

（11）Translate into English100MLStop adding liquid to each micropore

（12）After adding the termination solution30Measurement within minutes450nm(Reference wavelength:600-650nm）Absorbance at [location]

【 Calculation Results 】】

Based on the standard concentrationXThe corresponding absorbance of the axis isYAxis construction standard curve (such as double logarithmic quadratic regression fitting)

The sample concentration can be directly read from the standard curve

When calculating the sample concentration, it is necessary to multiply it by the corresponding dilution factor

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