Pertussis toxin IgG antibody detection kit

Pertussis toxin IgG antibody detection kit

Common name: Pertussis toxinIgGDetection kit (enzyme-linked immunosorbent assay)

English name:Bordetella PT IgG ELISA

96T

The reagent kit can be used for the detection of pertussis toxin in human serum and plasma (citrate, heparin)IgGQuantitative testing.

【 Detection Principle 】

This solid phasePertussis toxin IgG antibody detection kitThe sandwich method is used. The micropores are coated with antigens, which can be used to treat humansIgGThere are specific enzyme-linked antibodies to detect specific antibodies in the sample (which can bind to the coated antigen). After the substrate reaction, the intensity of the displayed color is compared to the detected colorIgGThe amount of specific antibodies is proportional. The results of the sample can be obtained directly using the standard curve.

Inspection steps

1)plus100µLreference standardQuality control productsDiluted sampletoIn the corresponding hole,A1For blank.

2)Room temperature after sealing（37℃）Nurture and nurture60minute

3)Remove the sealing paper and discard the liquid in the hole.300µL/KongWash the board with detergent3Next, pat dry on absorbent paper.(Machine wash)350µL/Hole,5Next time)

4)join100µL/KongEnzyme linked compounds，A1not add.

5)Room temperature after sealing（20℃-25℃）light-proofNurture and nurture30minute

6)Repeat steps3

7)plus100µL/KongTMBSubstrate solution.

8)Room temperature（20℃-25℃）Avoid light and incubate15minute

9)Add to each micropore100µLTMBTerminate the substrate reaction with the termination solution. Gently shake the microplate to mix the reagents evenly. At this point, the color blue turns yellow.

10)plus100µL/KongAfter terminating the liquid，in30within minutesYu450/620 nmTest locationODValue.

【 Result Calculation 】

In order for the analysis method to be effective, the following criteria must be met:§

blankA1absorbance value<0.100§

reference standardAabsorbance value<0.200§

reference standardBabsorbance value>reference standardA§

reference standardCabsorbance value>reference standardB§

reference standardDabsorbance value>reference standardC§

reference standardEabsorbance value>1.000§

Low quality control resultsIU/mLWithin the scope of the label, §

High quality control resultsIU/mLWithin the range displayed on the label

reference standardA<reference standardB<reference standardC<reference standardD<reference standardE

If these standards are not met, the test is invalid and must be repeated

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