Rat GRO/CINC-1 detection kit

Rat GRO/CINC-1 detection kit

【intended use】

This reagent kitCan be used for quantitative detection of rat serumEDTA-In plasma and cell supernatantGRO/CINC-1Restructuring and Natural FormsGRO/CINC-1All can be detected. This product is only used for research and not for diagnosis.

[Introduction]

Cytokine induced neutrophil chemotactic agent-1（CINC-1）Initially, it was fromIL-1β - stimulated rat renal epithelial like cells（NRK-52E）Purified from the culture medium under certain conditions. The research team from Toyama Medical and Pharmaceutical University in Watanabe1989The coding rat was determined in the yearCINC-1The amino acid sequence.CINC-1It's a chemokinealpha（CXC）A member of the Ya family. Three other types of ratsCXCChemokines（CINC-2and α,CINC-2and β,CINC-3/MIP-2）It has been identified.CINC-1The protein sequence andCINC-2a,CINC-2and β,CINC-3/MIP-2The protein sequence homology is63 - 67%In addition,GROa,GROβ andGROGamma andCINC-1The consistency is as follows:68%、71%and69%This already indicates that,CINCshuman beingsGROsThe rat counterpart.

【Detection Principle】

Rat GRO/CINC-1 detection kitBased on the principle of solid-phase sandwich method, two highly specific antibodies are used for detection. Tetramethylbenzidine（TMB）As a chromogenic reagent, the color intensity is comparable to that of ratsGRO/CINC-1The quantity is directly proportional.

【Kit components】

1）Microplate: 96Kong, coated anti ratGRO/CINC-1rabbitIgGAffinity purification

2）Labeling antibodies: 30Double concentration,1x0.4ml，HRP-anti-ratGRO/CINC-1rabbitIgG Fab’Affinity purification

3）Standard product: 2x0.5mlRecombinant ratsGRO/CINC-1

4）EIABuffer solution: 1x30ml

5）Labeling antibody solution: 1x12ml

6）Substrate solution: 1x15ml

7）Termination solution: 1x12ml

8）Wash buffer concentrate: 1x50ml，40Double concentration

【store】

The reagent kit should be stored in2-8At ℃, the expiration date should be indicated on the reagent kit, and expired reagents should not be used.

【Preparation of reagents】

（1）Wash buffer solution

Balance to room temperature before use and gently mix evenly

50mLWash buffer concentrate+1950mLDistilled water is mixed evenly to obtain the washing buffer working solution. The diluted washing buffer solution can be stored in the refrigerator2week

（2）Labeling antibodies

Labeling antibody solution in disposable test tubes30Diluted labeled antibody concentrate, if used1Micro porous plate（8Hole), needed800MLLabeling antibodies, i.e30MLLabel antibody concentrate+870MLLabel antibody solution and mix evenly

Preparation should only be carried out before use, and the labeled antibody concentrate should be prepared before use4Sealed storage at ℃

（3）Standard product

Add to standard samples0.5mLDissolve it in distilled water to obtain a standard concentration of600pg/mL.

prepare8Add test tubes and add them to each tube230ML EIAbuffer solution

Translate into English230MLAdd the reconstructed standard into the test tube1Gently mix well; Translate into English230MLtest tube1Add the medium solution into the test tube2Mix evenly. continuous2Double dilution preparation7The concentration of a standard substance is within the range of300 - 4.69pg/mL, test tube8The concentration is0pg/mL, as a sample blank

You can leave us a message on the platform, or follow the official account of "Kerunda Biology"