RSR aquaporin 4 antibody ELISA detection kit

NegotiableUpdate on 05/06

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Overview

RSR Aquaporin 4 Antibody ELISA Detection Kit $n Neuroimmune Related Antibody Detection-AQP4 Antibody is used for quantitative detection of Aquaporin 4 autoantibodies (AQP4 Ab) in human serum, and is suitable for diagnosing Neuromyelitis Optic (NMO), also known as Devic's disease and related disease spectrum (NMOSD). Detecting AQP4 Ab can also help in early differential diagnosis of NMO and multiple sclerosis (MS), which is beneficial for early intervention and treatment of NMO. This kit uses highly purified AQP4 M23 protein

Product Details

　　RSR aquaporin 4 antibody ELISA detection kit

　　Neuroimmune related antibody detection - AQP4 antibody（Registration Certificate Number: National Medical Products Administration 20192400461

　　1. Background

Neuromyelitis optica (NMO), also known as Devic's syndrome, is a neuroimmune disease mediated by an immune response that accumulates in the optic nerve and spinal cord. Neuromyelitis optica spectrum disorders (NMOSD), in addition to classic optic neuritis, also include non optic neuritis or myelitis, clinically manifested as optic neuritis or myelitis, including segmental transverse myelitis or isolated optic neuritis. There is a specific standard substance in the serum of NMO or NMOSD patients - immunoglobulin G autoantibody (NMO IgG). This autoantibody is mainly composed ofAquaporin 4 (AQP4)As an antigen.

　　2. Clinical significance

detectionAQP4 antibodyCan be used for:

A: Optic neuromyelitis and is an auxiliary diagnosis for neuromyelitis spectrum diseases;

B: Diagnosis of demyelinating patients before treatment;

C: Follow up monitoring of patients with optic neuritis and myelitis spectrum;

D: Differential diagnosis between optic neuritis myelitis and optic neuritis myelitis spectrum diseases and multiple sclerosis (MS).

　　3. Detection principle

　　Neuroimmune related antibody detection - AQP4 antibodyThe detection kit adopts bridge enzyme-linked immunosorbent assay technology, which enables the antibody (AQP4 Ab) in the test sample to simultaneously bind to the antigen (AQP4) encapsulated in the micropores and the biotinylated antigen (AQP4 biotin). By detecting the bound AQP4 biotin through enzyme-linked immunosorbent assay, the concentration of AQP4 Ab in the test sample can be calculated.

　　4. Testing process

1) Add standard quality control samples;

2) Add dissolved AQP4 biotin;

3) Incubate at room temperature with shaking for two hours;

4) Wash the board

5) Add diluted enzyme conjugates;

6) Shake incubation

7) Wash the board

8) Add chromogenic substrate

9) Incubate in the dark

10) Add termination solution

11) Read the absorbance values using 450nm and 405nm wavelengths respectively

　　5.characteristic

Sensitivity: 80%, in 83 patients with optic neuritis and myelitis; 100% of 64 patients with optic neuritis and myelitis spectrum diseases tested positive for NMO IgG in their serum.

Specificity: 99%, among 358 healthy individuals;

Standard range: 1.5-80 units/mL (RSR defined concentration);

Critical value: Negative:<3 units/mL; Positive: ≥ 3 units/mL;

　　6. Application scope

It can be conducted in clinical laboratories at all levels of hospitals and can be used in conjunction with their automated systems.

　　7. Applicable instruments

ELISA reader (wavelength 405nm, 450nm).

　　8. Product specifications

96 tests per box.

　　9. Storage conditions and expiration date

Stored at 2-8 ℃, with a shelf life of 9 months.