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RSR AQP4Ab Kit ELISA NMOSD

NegotiableUpdate on 05/06

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Overview

The RSR AQP4Ab kit ELISA NMOSD is used to determine the content in serum, plasma, and related liquid samples. There are also expression assay kits used to determine indicators in serum, plasma, and related liquid samples. If you need more Elisa reagent kit products, please call for more information. The reagent kit should be operated strictly according to the instructions, and different batches of reagents should not be mixed.

Product Details

RSR AQP4Ab Kit ELISA NMOSD

Neuroimmune related antibody detection - AQP4 antibody (registration certificate number: National Medical Products Administration 20192400461)

1. Background

Neuromyelitis optica (NMO), also known as Devic's syndrome, is a neuroimmune disease mediated by an immune response that accumulates in the optic nerve and spinal cord. Neuromyelitis optica spectrum disorders (NMOSD), in addition to classic optic neuritis, also include non optic neuritis or myelitis, clinically manifested as optic neuritis or myelitis, including segmental transverse myelitis or isolated optic neuritis. There is a specific standard substance in the serum of NMO or NMOSD patients - immunoglobulin G autoantibody (NMO IgG). The main antigen of this autoantibody is aquaporin 4 (AQP4).

2. Clinical significance

Detecting AQP4 antibodies can be used for:

A: Optic neuromyelitis and is an auxiliary diagnosis for neuromyelitis spectrum diseases;

B: Diagnosis of demyelinating patients before treatment;

C: Follow up monitoring of patients with optic neuritis and myelitis spectrum;

D: Differential diagnosis between optic neuritis myelitis and optic neuritis myelitis spectrum diseases and multiple sclerosis (MS).

3. Detection principle

The neuroimmune related antibody detection AQP4 antibody detection kit adopts bridge enzyme-linked immunosorbent assay technology, which enables the antibody (AQP4 Ab) in the test sample to simultaneously bind to the antigen (AQP4) encapsulated in the micropores and the biotinylated antigen (AQP4 biotin). By detecting the bound AQP4 biotin through enzyme-linked immunosorbent assay, the concentration of AQP4 Ab in the test sample can be calculated.

4. Testing process

1) Add standard quality control samples;

2) Add dissolved AQP4 biotin;

3) Incubate at room temperature with shaking for two hours;

4) Wash the board

5) Add diluted enzyme conjugates;

6) Shake incubation

7) Wash the board

8) Add chromogenic substrate

9) Incubate in the dark

10) Add termination solution

11) Read the absorbance values using 450nm and 405nm wavelengths respectively

5. Characteristics

Sensitivity: 80%, in 83 patients with optic neuritis and myelitis; 100% of 64 patients with optic neuritis and myelitis spectrum diseases tested positive for NMO IgG in their serum.

Specificity: 99%, among 358 healthy individuals;

Standard range: 1.5-80 units/mL (RSR defined concentration);

Critical value: Negative:<3 units/mL; Positive: ≥ 3 units/mL;

6. Application scope

It can be conducted in clinical laboratories at all levels of hospitals and can be used in conjunction with their automated systems.

7. Applicable instruments

ELISA reader (wavelength 405nm, 450nm).

8. Product specifications

96 tests per box.

9. Storage conditions and expiration date

Stored at 2-8 ℃, with a shelf life of 9 months.

Precautions for using RSR AQP4Ab kit:

Before testing, check various instruments, turn on the enzyme-linked immunosorbent assay reader, and stabilize for about 20 minutes.

2. The test sample should be clear and free of impurities, otherwise it will directly affect the results.

Before the experiment begins, the reagent kit should be taken out and left at room temperature for 30-40 minutes to allow all reagents to return to room temperature, in order to make the detection results more stable.

4. Try to conduct double standard experiments as much as possible to ensure the accuracy of the data.

5. The substrate is toxic and the termination solution is corrosive to the skin, so contact should be avoided.

In the experiment, the substrate should be stored away from light. The enzyme-linked immunosorbent assay (ELISA) plate can be disassembled, and any excess parts should be sealed and stored at low temperature.