Q ExactivePlus Q Exactive HF High Resolution Mass Spectrometer

NegotiableUpdate on 06/26

Model

Nature of the Manufacturer: Producers

Product Category

Place of Origin

Online Consult

Overview

The Q ExactivePlus Q Exactive HF high-resolution mass spectrometer combined with a quadrupole Orbitrap mass spectrometer can accurately identify and quantify more proteins, peptides, lipids, polysaccharides, and small molecules in less time. The Q Exactive HF system combines advanced segmented quadrupole technology for high-performance parent ion selection with a high-resolution, precise mass number (HR/AM) ultra-high field Orbitrap mass analyzer, achieving an excellent combination of scanning speed, resolution, mass accuracy, spectral quality, and sensitivity.

Product Details

Q ExactivePlus Q Exactive HF High Resolution Mass SpectrometerThe ultra-high electrostatic field orbit trap Fourier transform bench mass spectrometry combines high-performance quadrupole rods that provide parent ion selection and ultra-high electrostatic field orbit traps with ultra-high resolution and high-quality accuracy. It has shown some performance in applications such as peptides, proteins, and small molecules with faster detection speed and higher resolution.

The Thermo Fisher LCMS liquid chromatography-mass spectrometry system Q Exactive HF adds an ultra-high field Orbitrap mass analyzer to the validated Q Exactive Plus LC-MS system. This system is designed for faster identification, characterization, quantification, and confirmation in a highly reliable analysis; In order to significantly improve analysis efficiency, the maximum scanning speed has been increased by 50%. The Q Exactive HF system can complete dozens of complete MSMS scans in 1 second.

Q Exactiveplus Q Exactive HF高分辨质谱仪

Q ExactivePlus Q Exactive HF High Resolution Mass SpectrometerThe Orbitrap used in Fusion is an ultra-high field Orbitrap mass analyzer. Compared to other Orbitrap series products from Thermo Fisher, Fusion has ultra-high resolution, high sensitivity, multi-stage mass spectrometry capabilities, and is equipped with multiple fragmentation modes (CID, HCD, and upgradable ETD), making it very suitable for high-throughput protein detection in complex systems in proteomics.

Quality accuracy and stability

Quality accuracy

Q Exactive Plus: Internal standard<1 ppm RMS; External standard<3 ppm RMS
Q Exactive HF: Internal standard<1 ppm RMS; External standard<3 ppm RMS

quality stability

After one calibration, it can remain stable for 48-72 hours, and the accuracy of the external standard method is ≤ 5 ppm

Sensitivity and dynamic range

Sensitivity (ESI+)

Under the same conditions, the sensitivity is increased by about 30% -50% compared to Plus
Full MS：500 fg ，S/N>100:1
SIM：30 fg ，S/N>100:1
Q Exactive Plus
Q Exactive HF

Dynamic range:>5000:1 (full scan)

Three ion source and transport system

Ion source: standard HESI-II (electric spray) APCI； Optional MALDI
Polarity switching:<1 s (one positive+one negative full scan, resolution 35000)
Quadrupole pole: all metal molybdenum double curved surface, minimum isolation window 0.4 Da
Ion guidance

Q Exactive Plus：AABG（Advanced Active Beam Guide）
Q Exactive HF：AQT（Advanced Quadrupole Technology）+ AABG

Four fragmentation mode and collection capability

Fragmentation method: HCD (high-energy collision dissociation), CID (optional)
Data Dependent Acquisition (DDA): Up to 10 parent ions can be scanned in a single scan
Acquisition modes: Full MS, SIM, PRM, DIA, MS/MS

Five Physical and Environmental Parameters

Dimensions (width x depth x height): 950 x 830 x 910 mm
Weight: Approximately 270 kg
Vacuum system: Molecular turbo pump, orbital trap vacuum ≤ 1 × 10 ⁻¹⁰ mbar

Six core differences and selection suggestions

Resolution: HF (240k)>Plus (140k), priority given to complex matrix/low abundance analysis HF
Speed: HF (18 Hz)>Plus (12 Hz), suitable for high-throughput omics
Sensitivity: Higher HF, suitable for trace and biopharmaceutical analysis

Q Exactiveplus Q Exactive HF高分辨质谱仪